Kosmala Monika, Zduńczyk Zenon, Kołodziejczyk Krzysztof, Klimczak Elżbieta, Juśkiewicz Jerzy, Zduńczyk Przemysław
Institute of Chemical Technology of Food, Lodz University of Technology, ul. B. Stefanowskiego 4/10, 90-924, Lodz, Poland,
Eur J Nutr. 2014;53(2):521-32. doi: 10.1007/s00394-013-0557-z. Epub 2013 Jul 12.
The objective of this study was to establish the composition of polyphenolic preparations obtained from industrial strawberry pomace with two methods of extraction: the water and the water-alcoholic one and then to analyse their effects in the gastrointestinal tract depending on the composition of dietary fibre-cellulose or fructooligosaccharides (FOS).
Freeze-dried water extract (PTW), containing 5.1% of ellagic acid, 0.2% of proanthocyanidins, and soluble carbohydrates as a major part, and water-alcohol extract (PTE), containing 17.1% of ellagic acid and 10.9% of proanthocyanidins, were administered, in the equivalent quantity of 0.06% of ellagic acid, to 4- to 8-week-old rats (8 animals per group), as a component of modified AIN-93 diets containing 5% of cellulose or FOS.
The addition of strawberry pomace extracts had no effect on either the diet intake or the body weight of experimental rats. Both extracts, similarly to FOS, beneficially reduced the activity of β-glucuronidase in caecal digesta, with the PTW effect being significantly higher than that of PTE (7.59 vs. 9.20 μmol/h/g, P = 0.001). In comparison with PTE, the PTW extract significantly increased the caecal digesta mass (1.45 vs. 1.27 k/kg BW) and the total production of SCFA (86.1 vs. 71.4 μmol/100 g BW). The extract enhanced the physiological effect of FOS by inhibiting the activity of β-glucuronidase, increasing the caecal digesta mass and SCFA production. Such an effect was not recorded in the case of the PTE preparation.
The addition of strawberry pomace extracts affected the activity of certain enzymes of intestinal microflora and its most important products.
本研究的目的是确定通过两种提取方法（水提法和水 - 醇提法）从工业草莓果渣中获得的多酚制剂的成分，然后根据膳食纤维（纤维素或低聚果糖（FOS））的组成分析它们在胃肠道中的作用。
将含有5.1％鞣花酸、0.2％原花青素且主要成分为可溶性碳水化合物的冻干水提取物（PTW）和含有17.1％鞣花酸和10.9％原花青素的水 - 醇提取物（PTE），以相当于0.06％鞣花酸的量，给予4至8周龄的大鼠（每组8只动物），作为含有5％纤维素或FOS的改良AIN - 93饮食的成分。
添加草莓果渣提取物对实验大鼠的饮食摄入量或体重均无影响。与FOS类似，两种提取物均有益地降低了盲肠消化物中β - 葡萄糖醛酸酶的活性，PTW的效果显著高于PTE（7.59对9.20μmol/h/g，P = 0.001）。与PTE相比，PTW提取物显著增加了盲肠消化物质量（1.45对1.27 k/kg体重）和短链脂肪酸的总产量（86.1对71.4μmol/100 g体重）。该提取物通过抑制β - 葡萄糖醛酸酶的活性、增加盲肠消化物质量和短链脂肪酸的产生，增强了FOS的生理作用。在PTE制剂中未观察到这种效果。
添加草莓果渣提取物影响了肠道微生物群某些酶的活性及其最重要的产物。
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