Ansari Mohammad Israil, Yadav Ashok, Lal Ramji
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Gomti Nagar, Lucknow-226 010, India.
Bioinformation. 2013 May 25;9(9):464-5. doi: 10.6026/97320630009464. Print 2013.
Saccharum officinarum is one of the most cultivated hybrid varieties among the sugarcane varieties. In sugarcane plant sucrose is the major carbohydrate which can be stored and transported. Different physiological and biochemical studies on this crop report that invertase activity and sucrose concentration some how are key limiting step in the process of sucrose accumulation. Significant efforts have been made in relation to the sucrose cycle by altering the sucrose phosphate synthetase, sucrose synthetase and invertase. In sugarcane two types of invertase enzymes have been reported on the basis of pH and cellular localization. Invertase breaks the sucrose into hexoses as a source of energy and carbon. It has also been reported that this enzyme is involved in the process of cell differentiation and plant development. Progress has been made for the understanding of invertase activity and its role in sugarcane plant. With the help of biotechnology it is possible to target the desired gene with genetic engineering approach to increase sucrose content by careful manipulation of invertase (enzyme) gene to increase the sucrose yield in sugarcane. Purpose of this mini review is to high-light the role of invertase in sugarcane and how to overcome sucrose recovery in sugarcane.
甘蔗是甘蔗品种中种植最广泛的杂交品种之一。在甘蔗植株中,蔗糖是主要的可储存和运输的碳水化合物。对这种作物进行的不同生理生化研究表明,转化酶活性和蔗糖浓度在某种程度上是蔗糖积累过程中的关键限制步骤。通过改变蔗糖磷酸合成酶、蔗糖合成酶和转化酶,在蔗糖循环方面已经做出了重大努力。在甘蔗中,根据pH值和细胞定位,已报道了两种类型的转化酶。转化酶将蔗糖分解为己糖,作为能量和碳的来源。也有报道称,这种酶参与细胞分化和植物发育过程。在了解转化酶活性及其在甘蔗植株中的作用方面已经取得了进展。借助生物技术,通过基因工程方法靶向所需基因,通过仔细操纵转化酶(酶)基因来增加蔗糖含量,从而提高甘蔗的蔗糖产量是可能的。本综述的目的是突出转化酶在甘蔗中的作用以及如何提高甘蔗的蔗糖回收率。