Integrated Bioscience Program, Department of Biology, University of Akron Akron, OH, USA.
Front Physiol. 2013 Jul 1;4:160. doi: 10.3389/fphys.2013.00160. eCollection 2013.
We used antisense morpholino oligonucleotide technology to knockdown leptin-(A) gene expression in developing zebrafish embryos and measured its effects on metabolic rate and cardiovascular function. Using two indicators of metabolic rate, oxygen consumption was significantly lower in leptin morphants early in development [<48 hours post-fertilization (hpf)], while acid production was significantly lower in morphants later in development (>48 hpf). Oxygen utilization rates in <48 hpf embryos and acid production in 72 hpf embryos could be rescued to that of wildtype embryos by recombinant leptin coinjected with antisense morpholino. Leptin is established to influence metabolic rate in mammals, and these data suggest leptin signaling also influences metabolic rate in fishes.
我们使用反义寡核苷酸技术敲低了发育中的斑马鱼胚胎中的瘦素(A)基因表达,并测量了其对代谢率和心血管功能的影响。使用代谢率的两个指标,瘦素敲低组在早期发育(受精后<48 小时)时的耗氧量显著降低,而在晚期发育(>48 hpf)时的产酸量显著降低。用重组瘦素与反义寡核苷酸共注射可以将<48 hpf 胚胎的耗氧率和 72 hpf 胚胎的产酸率恢复到野生型胚胎的水平。瘦素在哺乳动物中被确定为影响代谢率的因素,这些数据表明瘦素信号也影响鱼类的代谢率。