Institute for Lasers, Life and Biophotonics, Faculty of Sciences, VU University Amsterdam , De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands.
J Phys Chem B. 2013 Sep 26;117(38):11363-71. doi: 10.1021/jp4031283. Epub 2013 Jul 30.
Time-resolved fluorescence spectroscopy measurements at 77 K on thylakoid membrane preparations and isolated photosynthetic complexes thereof were investigated using target analysis with the aim of building functional compartmental models for the photosystems in the thylakoid membrane. Combining kinetic schemes with different spectral constraints enabled us to resolve the energy transfer pathways and decay characteristics of the different emissive species. We determined the spectral and energetic properties of the red Chl pools in both photosystems and quantified the formation of LHCII-LHCI-PSI supercomplexes in the transition from native to unstacked thylakoid membranes.
在 77 K 下对类囊体膜制剂和分离的光合复合物进行了时间分辨荧光光谱测量，使用目标分析进行了研究，目的是为类囊体膜中的光合作用系统构建功能隔室模型。将动力学方案与不同的光谱约束相结合，使我们能够解析不同发射物种的能量转移途径和衰减特征。我们确定了两个光系统中红色 Chl 池的光谱和能量特性，并量化了在从天然到未堆叠类囊体膜的转变中 LHCII-LHCI-PSI 超复合物的形成。
