基于高分辨率序列的DPA1分型从肯尼亚人群中鉴定出两个新的DPA1等位基因，即DPA1010303和DPA10303。

High-resolution sequence-based DPA1 typing identified two novel DPA1 alleles, DPA1010303 and DPA10303, from a Kenyan population.

作者信息

Luo M, Bamforth J, Gill K, Cohen C, Brunham R C, Plummer F A

机构信息

Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.

出版信息

Tissue Antigens. 2005 Jan;65(1):120-2. doi: 10.1111/j.1399-0039.2005.00339.x.

DOI:10.1111/j.1399-0039.2005.00339.x

PMID:15663751

Abstract

We report here two novel DPA1 alleles, DPA1010303 and DPA10303, identified from a Kenyan population during sequence-based HLA-DPA1 typing. Molecular cloning and sequencing of multiple clones confirmed that one of the new DPA1 alleles is identical to DPA1010301 at exon 2, except for a single nucleotide substitution (ACG ACC) at codon 15. The new allele has been named by the WHO Nomenclature Committee as DPA1010303. The second novel DPA1 allele is identical to DPA10301, except for a single nucleotide difference (GAA GAC) at codon 28 that changed the amino acid from Glu to Asp. The new allele has been named by the WHO Nomenclature Committee as DPA10303. Identification of the two novel DPA1 alleles reflects the genetic diversity of this East African population.

摘要

我们在此报告在基于序列的HLA-DPA1分型过程中从肯尼亚人群中鉴定出的两个新的DPA1等位基因，即DPA1010303和DPA10303。对多个克隆进行分子克隆和测序证实，其中一个新的DPA1等位基因在第2外显子上与DPA1010301相同，只是密码子15处有一个单核苷酸替换（ACG→ACC）。该新等位基因已被世界卫生组织命名委员会命名为DPA1010303。第二个新的DPA1等位基因与DPA10301相同，只是密码子28处有一个单核苷酸差异（GAA→GAC），导致氨基酸从Glu变为Asp。该新等位基因已被世界卫生组织命名委员会命名为DPA10303。这两个新的DPA1等位基因的鉴定反映了这个东非人群的遗传多样性。