巴西女性的乳汁含有分泌型 IgA 抗体，可中和轮状病毒 G9P[5]。
Milk from Brazilian women presents secretory IgA antibodies and neutralizes rotavirus G9P[5].
机构信息
MD. Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, Brazil.
出版信息
J Pediatr (Rio J). 2013 Sep-Oct;89(5):510-3. doi: 10.1016/j.jped.2013.02.017. Epub 2013 Jul 10.
OBJECTIVE
To verify the presence of anti-rotavirus serotype G9P[5] SIgA and the virus neutralization capacity of milk samples from Brazilian women.
METHODS
SIgA antibody levels reactive to rotavirus G9 were determined in 30 maternal milk samples by enzyme-linked immunosorbent assay (ELISA) using purified virus suspensions. The samples' capacity to neutralize rotavirus G9P[5] was analyzed using the MA-104 cells neutralization assay.
RESULTS
Great individual variations were observed regarding the SIgA levels and neutralization titers, but all samples showed some G9P[5] neutralizing ability. A highly significant positive correlation was observed between antibody levels and neutralization titers.
CONCLUSIONS
The high correlation between anti-rotavirus antibody levels and neutralizing capacity of the milk samples suggests a possible protective role of these antibodies against infection. These results also support the encouragement of the breast-feeding practice.
目的
验证巴西妇女母乳样本中抗轮状病毒血清型 G9P[5] SIgA 的存在和病毒中和能力。
方法
采用酶联免疫吸附试验（ELISA）法，用纯化的病毒悬液检测 30 份母乳样本中针对轮状病毒 G9 的 SIgA 抗体水平。采用 MA-104 细胞中和试验分析样本对轮状病毒 G9P[5]的中和能力。
结果
SIgA 水平和中和滴度存在很大的个体差异，但所有样本均显示出一定的 G9P[5]中和能力。抗体水平与中和滴度之间存在高度显著的正相关。
结论
母乳样本中抗轮状病毒抗体水平与中和能力之间的高度相关性表明这些抗体可能对感染具有保护作用。这些结果也支持鼓励母乳喂养。
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