Tino De Franco M, Vieira P D, Santos S M R, Ferreira T L, Araújo E D, Quintal V S, Carbonare S B
Immunogenetics Laboratory, Butantan Institute, São Paulo, Brazil.
Paediatr Int Child Health. 2013 May;33(2):102-7. doi: 10.1179/2046905513Y.0000000057.
Rotavirus is an important aetiological agent for severe diarrhoea in infants and young children worldwide. Anti-rotavirus antibodies in human colostrum and milk may interfere with rotavirus vaccination seroconversion.
To verify the presence of anti-rotavirus secretory IgA antibodies (SIgA) and the neutralizing capacity of 30 colostrum and 30 milk samples from Brazilian women in two different centres and analyze their persistence throughout lactation.
Colostrum and milk samples from healthy nursing mothers were tested for the presence of anti-rotavirus SIgA using conventional ELISA and their capacity to neutralize rotavirus using MA-104 cell cultures. Total IgA concentrations and anti-rotavirus SIgA levels were measured in samples collected from three mothers during 90 or 240 days of the lactation period.
Colostrum samples showed higher levels of anti-rotavirus SIgA and higher neutralizing ability than in milk. However, these antibodies levels were not statistically different. In addition, there was no correlation between antibody levels and the neutralizing activity observed in colostrum and milk samples. Follow-up of three mothers demonstrated the persistence of anti-rotavirus and total IgA levels throughout lactation.
These results support the encouragement of breastfeeding as a mechanism of protection against rotavirus infection in lactating infants. Components other than SIgA antibodies might play an important role in virus neutralization.
轮状病毒是全球婴幼儿严重腹泻的重要病原体。人初乳和乳汁中的抗轮状病毒抗体可能会干扰轮状病毒疫苗接种后的血清转化。
验证来自巴西两个不同中心的30份初乳和30份乳汁样本中抗轮状病毒分泌型IgA抗体（SIgA）的存在及其中和能力，并分析其在整个哺乳期的持续性。
使用传统酶联免疫吸附测定法检测健康哺乳期母亲的初乳和乳汁样本中抗轮状病毒SIgA的存在情况，并使用MA-104细胞培养法检测其中和轮状病毒的能力。在哺乳期90天或240天内从三位母亲采集的样本中测量总IgA浓度和抗轮状病毒SIgA水平。
初乳样本中抗轮状病毒SIgA水平和中和能力高于乳汁。然而，这些抗体水平在统计学上没有差异。此外，初乳和乳汁样本中观察到的抗体水平与中和活性之间没有相关性。对三位母亲的随访表明，整个哺乳期抗轮状病毒和总IgA水平持续存在。
这些结果支持鼓励母乳喂养，作为哺乳期婴儿预防轮状病毒感染的一种保护机制。除SIgA抗体外的其他成分可能在病毒中和中起重要作用。
