National Institute of Crop Science, RDA, Suwon, Republic of Korea.
J Virol Methods. 2013 Nov;193(2):674-8. doi: 10.1016/j.jviromet.2013.07.008. Epub 2013 Jul 11.
Rice stripe virus (RSV), Rice black-streaked dwarf virus (RBSDV), and Rice dwarf virus (RDV) are major rice-infecting viruses in Korea that can cause serious crop losses. A one-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for the simultaneous detection of these rice viruses. Three sets of specific primers targeted to the capsid protein coding genes of RSV, RBSDV, and RDV were used to amplify fragments that were 703 bp, 485 bp, and 252 bp, respectively. The one-step mRT-PCR assay proved to be a sensitive and rapid method for detecting the three rice viruses. This method could be used to facilitate better control of rice viruses.
水稻条纹病毒（RSV）、水稻黑条矮缩病毒（RBSDV）和水稻矮缩病毒（RDV）是韩国主要侵染水稻的病毒，可导致严重的作物损失。本研究建立了一种一步法多重逆转录聚合酶链反应（mRT-PCR），用于同时检测这些水稻病毒。该方法使用针对 RSV、RBSDV 和 RDV 外壳蛋白编码基因的三组特异性引物，分别扩增出 703 bp、485 bp 和 252 bp 的片段。一步法 mRT-PCR 检测方法灵敏、快速，可用于检测三种水稻病毒。该方法有助于更好地控制水稻病毒。
