Institute of Molecular Biology and Biophysics, Swiss Federal Institute of Technology (ETH Zürich), Zürich, Switzerland.
Nat Struct Mol Biol. 2013 Aug;20(8):1015-7. doi: 10.1038/nsmb.2622. Epub 2013 Jul 14.
Eukaryotic translation initiation factors (eIFs) 1A and 1 are central players in the complex process of start-codon recognition. To improve mechanistic understanding of this process, we determined the crystal structure of the 40S ribosomal subunit in complex with eIF1A and eIF1 from Tetrahymena thermophila at a resolution of 3.7 Å. It reveals the positions of the two factors on the 40S and the conformational changes that accompany their binding.
真核翻译起始因子 (eIFs) 1A 和 1 是起始密码子识别这一复杂过程的核心参与者。为了深入了解这一过程的机制，我们以 3.7Å 的分辨率测定了嗜热四膜虫的 40S 核糖体亚基与 eIF1A 和 eIF1 复合物的晶体结构。该结构揭示了这两个因子在 40S 上的位置以及伴随它们结合的构象变化。
