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一种通过筛选肿瘤 cDNA 表达文库与癌症血清反应来鉴定肿瘤抗原的微阵列方法。

A microarray method for identifying tumor antigens by screening a tumor cDNA expression library against cancer sera.

机构信息

Center for Innovations in Medicine; The Biodesign Institute; Arizona State University; Tempe, AZ USA; Biological Design Program; Arizona State University; Tempe, AZ USA.

Center for Innovations in Medicine; The Biodesign Institute; Arizona State University; Tempe, AZ USA; HealthTell, Inc.; Chandler, AZ USA.

出版信息

Hum Vaccin Immunother. 2013 Oct;9(10):2178-88. doi: 10.4161/hv.25634. Epub 2013 Jul 12.

DOI:10.4161/hv.25634
PMID:23851590
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3906402/
Abstract

The immune system responds to tumor cells. The challenge has been how to effectively use these responses to treat or protect against cancer. Toward the goal of developing a cancer vaccine, we are pursuing methodologies for the discovery and testing of useful antigens. We present an array-based approach for discovering these B cell antigens by directly screening for specific host-sera reactivity to lysates from tumor-derived cDNA expression libraries. Several cancer-specific antigens were identified, and these are currently being validated as potential candidates.

摘要

免疫系统对肿瘤细胞作出反应。挑战在于如何有效地利用这些反应来治疗或预防癌症。为了开发癌症疫苗，我们正在探索用于发现和测试有用抗原的方法。我们提出了一种基于阵列的方法，通过直接筛选肿瘤衍生 cDNA 表达文库的裂解物与宿主血清的特异性反应来发现这些 B 细胞抗原。已经鉴定出了几个癌症特异性抗原，目前正在对其进行验证，作为潜在的候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/66fddd84ae36/hvi-9-2178-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/2483d8cd64b9/hvi-9-2178-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/0fdf3173b862/hvi-9-2178-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/66fddd84ae36/hvi-9-2178-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/2483d8cd64b9/hvi-9-2178-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/0fdf3173b862/hvi-9-2178-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8561/3906402/66fddd84ae36/hvi-9-2178-g3.jpg

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