Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, China.
Ann Surg Oncol. 2013 Dec;20 Suppl 3:S644-9. doi: 10.1245/s10434-013-3132-1. Epub 2013 Jul 13.
SHOX2 (short stature homeobox 2) is a crucial transcriptional regulator in several genetic disorders and has been demonstrated to be an excellent biomarker in the diagnosis and evaluation of lung cancer. However, its expression pattern and prognostic value for hepatocellular carcinoma (HCC) are still unknown.
Expression of SHOX2 gene and protein in HCC tissues and cell lines were evaluated by RT-qPCR and western blot. Impact of RNAi-mediated SHOX2 silence on the proliferation and invasion ability of Huh7 cell line in vitro was determined by CCK-8 assay and matrigel invasion assay, respectively.
Elevated expression of SHOX2 gene was significantly associated with higher incidence of tumor recurrence (n = 60, p = 0.001), absence of tumor capsule (p = 0.015), presence of tumor thrombi (p < 0.0001), and advanced TNM stage (p < 0.0001) of HCC. SHOX2 protein expression was more abundant in HCC cell lines compared with hepatic cell line (p = 0.001), which was associated with tumor recurrence (n = 40, p = 0.046). RNAi-mediated silence of SHOX2 expression significantly inhibited the proliferation (p < 0.001) and invasion (p = 0.006) of Huh7 cell line in vitro.
Elevated SHOX2 expression was associated with HCC recurrence, probably by enhancing proliferation and invasion capability of cancer cells.
SHOX2（短肢同源盒 2）是几种遗传疾病中的关键转录调节因子，已被证明是诊断和评估肺癌的优秀生物标志物。然而，其在肝细胞癌（HCC）中的表达模式和预后价值尚不清楚。
通过 RT-qPCR 和 Western blot 评估 SHOX2 基因和蛋白在 HCC 组织和细胞系中的表达。通过 CCK-8 测定和基质胶侵袭测定分别确定 RNAi 介导的 SHOX2 沉默对 Huh7 细胞系体外增殖和侵袭能力的影响。
SHOX2 基因的高表达与肿瘤复发（n=60，p=0.001）、无肿瘤包膜（p=0.015）、存在肿瘤血栓（p<0.0001）和晚期 TNM 分期（p<0.0001）的 HCC 发生率显著相关。与肝细胞系相比，SHOX2 蛋白在 HCC 细胞系中的表达更为丰富（p=0.001），与肿瘤复发（n=40，p=0.046）相关。SHOX2 表达的 RNAi 介导沉默显著抑制 Huh7 细胞系的体外增殖（p<0.001）和侵袭（p=0.006）。
SHOX2 表达升高与 HCC 复发相关，可能通过增强癌细胞的增殖和侵袭能力。
