Department of Oncology, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000, China.
Department of Urology Surgery, Lishui People's Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University, No. 15 Dazhong Street, Liandu District, Lishui, 323000, China.
Biochem Genet. 2023 Oct;61(5):2004-2019. doi: 10.1007/s10528-023-10353-5. Epub 2023 Mar 14.
MiRNAs are essential epigenetic modulators that can regulate protein expression. According to the principle of base complementary pairing, miRNA is partially or completely complementary to the 3'-UTR region of its target gene, by which it inhibits the translation of the targeted gene. This study investigated the role of miR-24-1-5p in clear cell renal cell carcinoma (ccRCC). Data in TCGA-KIRC denoted that miR-24-1-5p was under-expressed in ccRCC. Bioinformatics analysis predicted that its target gene was SHOX2, which was significantly expressed in cancer tissues. Dual luciferase assay verified the targeting relationship between miR-24-1-5p and SHOX2. Cell function experiments demonstrated that overexpression of miR-24-1-5p significantly inhibited SHOX2 level and the malignant phenotypes of ccRCC cells. The above results illustrated that miR-24-1-5p/SHOX2 axis was critical for the oncogenesis and development of ccRCC, which might be helpful for us to understand the mechanism and novel therapeutic methods of ccRCC.
miRNAs 是重要的表观遗传调节剂，可以调节蛋白质的表达。根据碱基互补配对原则，miRNA 与靶基因的 3'-UTR 区域部分或完全互补，从而抑制靶基因的翻译。本研究探讨了 miR-24-1-5p 在透明细胞肾细胞癌（ccRCC）中的作用。TCGA-KIRC 中的数据表明，miR-24-1-5p 在 ccRCC 中表达下调。生物信息学分析预测其靶基因是 SHOX2，在癌组织中显著表达。双荧光素酶报告基因实验验证了 miR-24-1-5p 与 SHOX2 之间的靶向关系。细胞功能实验表明，miR-24-1-5p 的过表达显著抑制了 SHOX2 水平和 ccRCC 细胞的恶性表型。上述结果表明，miR-24-1-5p/SHOX2 轴在 ccRCC 的发生发展中起着关键作用，这可能有助于我们理解 ccRCC 的发病机制和新的治疗方法。
