Advanced Photonics Center, Southeast University, Nanjing 210096, China.
Small. 2013 Dec 20;9(24):4215-20. doi: 10.1002/smll.201301372. Epub 2013 Jul 15.
Telomerase is now considered to be a valuable biomarker and therapeutic target in the diagnosis and treatment of cancerous diseases, which brings an urgent need in the development of fast and efficient telomerase detection strategies. Here, a new surface enhanced Raman scattering (SERS) based protocol using telomeric elongation controlled SERS (TEC-SERS) effect for the ultrasensitive telomerase detection is presented. The TEC-SERS protocol not only provides an unprecedented high sensitivity but also avoids laborious PCR procedures. The detection limit is ≈2-3 orders of magnitude lower than those of previously reported methods. This highly sensitive and straightforward TEC-SERS protocol can be developed as a routine telomerase detection method, which would greatly facilitate the telomerase based ultra-early diagnosis of malignant tumors and the fast screening of anti-cancer drugs.
端粒酶现在被认为是癌症诊断和治疗中一种有价值的生物标志物和治疗靶点，这就迫切需要开发快速、高效的端粒酶检测策略。在这里，提出了一种新的基于表面增强拉曼散射（SERS）的协议，该协议使用端粒延伸控制的 SERS（TEC-SERS）效应进行超灵敏的端粒酶检测。TEC-SERS 协议不仅提供了前所未有的高灵敏度，而且避免了繁琐的 PCR 程序。检测限比以前报道的方法低约 2-3 个数量级。这种高灵敏度和简单的 TEC-SERS 协议可以开发为常规端粒酶检测方法，这将极大地方便基于端粒酶的恶性肿瘤超早期诊断和抗癌药物的快速筛选。
