Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering , East China Normal University , Dongchuan Road 500 , Shanghai 200241 , P. R. China.
ACS Sens. 2019 Jan 25;4(1):211-217. doi: 10.1021/acssensors.8b01244. Epub 2018 Dec 11.
Telomerase is a valuable biomarker, which is highly correlated to cancer diseases. However, the single-mode probe for telomerase detection cannot satisfy the challenge of detection of telomerase activity rapidly, simply with high selectivity, sensitivity, and accuracy both in preliminary diagnosis and in point of care (POC) testing. Therefore, there is an urgent need to develop a robust approach with controllable assembly and high accuracy to consider both the simplification of preliminary diagnosis and POC testing and the quantification requirement for early clinical diagnosis and treatment. Herein, a novel dual-mode Au NPs probe was developed for determination of telomerase activity with controllable assembly and aggregation statement based on surface enhancement Raman scattering (SERS) and colorimetry. In this strategy, an Au dimer-based probe with high uniformity was assembled successfully, telomerase activity was reflected according to the color variations of solution and the Raman intensity of Raman reporter. Taking advantage of the uniformity of Au dimers and the combination of colorimetry and SERS techniques, our strategy determined the telomerase activity with high accuracy, sensitivity, and wide range. The established probe possessed of high selectivity, sensitivity, and accuracy, which was approved as a reliable, intuitional, and convenient approach for detecting telomerase activity.
端粒酶是一种有价值的生物标志物，与癌症高度相关。然而，用于端粒酶检测的单模式探针不能满足快速、简单、具有高选择性、灵敏度和准确性的端粒酶活性检测的挑战，无论是在初步诊断还是在即时检测（POC）测试中。因此，迫切需要开发一种具有可控组装和高精度的稳健方法，既要考虑初步诊断和 POC 测试的简化，又要考虑早期临床诊断和治疗的定量要求。在此，基于表面增强拉曼散射（SERS）和比色法，开发了一种新型的双模式 Au NPs 探针，用于可控组装和聚集状态的端粒酶活性测定。在该策略中，成功组装了具有高均一性的基于 Au 二聚体的探针，根据溶液颜色变化和拉曼报告分子的拉曼强度来反映端粒酶活性。利用 Au 二聚体的均一性和比色法与 SERS 技术的结合，我们的策略以高精度、高灵敏度和宽范围确定了端粒酶活性。该探针具有高选择性、灵敏度和准确性，被证明是一种可靠、直观、方便的检测端粒酶活性的方法。
