Division of Rhinology&Sinus Surgery, Department of Otolaryngology-Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina.
Laryngoscope. 2013 Dec;123(12):E72-8. doi: 10.1002/lary.24305. Epub 2013 Aug 6.
OBJECTIVES/HYPOTHESIS: Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.
In vitro.
Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor-alpha (TNF-α), interferon-γ (IFN-γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one-way ANOVA with posthoc t test between groups.
Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL-8 and IFN-γ levels were outside of the detectable range of the assay. Levels of secreted IL-2, 4, 6, 10, and 17A correlated with tissue levels (P < 0.05 for all, r > 0.49) while TNF-α did not (P = 0.71). CRSsNP had elevated mucus levels of IL-2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL-4, 6, 10, 17A, and TNF-α compared to controls.
Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.
目的/假设：比较对照和慢性鼻-鼻窦炎患者窦组织和窦分泌物中的细胞因子水平。
体外。
将聚氨酯泡沫海绵置于无鼻息肉的慢性鼻-鼻窦炎患者（CRSsNP）、有息肉的慢性鼻-鼻窦炎患者（CRSwNP）和对照组的中鼻甲中。然后从同一部位采集鼻窦活检。通过流式细胞术检测每个样本的肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和白细胞介素（IL）2、4、6、8、10 和 17A 的蛋白水平。通过对样本之间的皮尔逊相关和组间的单向方差分析与事后 t 检验比较窦组织和分泌物中的蛋白值。
共检查了 43 名患者的样本。从 10 名对照、11 名 CRSsNP 和 10 名 CRSwNP 中测量了黏液，从 10 名对照、15 名 CRSsNP 和 10 名 CRSwNP 中测量了窦组织。IL-8 和 IFN-γ 水平超出了检测范围。分泌的 IL-2、4、6、10 和 17A 水平与组织水平相关（所有 P<0.05，r>0.49），而 TNF-α 则不相关（P=0.71）。CRSsNP 与对照相比，黏液中 IL-2、4、6、10 和 17A 水平升高。CRSwNP 与对照相比，黏液中 IL-4、6、10、17A 和 TNF-α 水平升高。
窦分泌物中的细胞因子水平与窦组织中的水平相关，基于 Th1/Th2 倾斜，CRS 比对照中的细胞因子水平升高。
