Department of Otolaryngology-Head and Neck Surgery, Medical University of South Carolina, Charleston, SC, USA.
Int Forum Allergy Rhinol. 2013 Aug;3(8):621-9. doi: 10.1002/alr.21173. Epub 2013 May 2.
Recent research suggest that B and plasma cells may play an important role in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). The purpose of this study was to subcharacterize the B cell response in the sinus mucosa of control and CRS patients.
Representative tissue samples and peripheral blood samples were obtained from controls, CRS without nasal polyps (CRSsNP) and CRSwNP. Using single-cell suspension flow cytometry these samples were analyzed for overall and stage-specific B and plasma cell percentages.
Both atopic and nonatopic CRSwNP patients showed an increase in local numbers of naive, active, and memory B cells compared to controls. CRSsNP patients only showed local elevations of naive B cells. Plasma cells were only significantly elevated in the sinus tissue of atopic CRSwNP patients. These local tissue increases did not correlate with increased numbers of circulating B cells.
This study provides further evidence of an important role of B cells in CRSwNP patients. The local increase appears to be independent of a systemic response.
最近的研究表明，B 细胞和浆细胞可能在慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）的发病机制中发挥重要作用。本研究的目的是对对照组和 CRS 患者的鼻窦黏膜中的 B 细胞反应进行细分。
从对照组、无鼻息肉的 CRS（CRSsNP）和 CRSwNP 患者中获得代表性的组织样本和外周血样本。使用单细胞悬浮流式细胞术分析这些样本的总体和阶段特异性 B 细胞和浆细胞百分比。
与对照组相比，特应性和非特应性 CRSwNP 患者的局部幼稚 B 细胞、活化 B 细胞和记忆 B 细胞数量均增加。CRSsNP 患者仅表现出局部幼稚 B 细胞升高。仅在特应性 CRSwNP 患者的鼻窦组织中浆细胞显著升高。这些局部组织的增加与循环 B 细胞数量的增加无关。
本研究进一步证明了 B 细胞在 CRSwNP 患者中的重要作用。局部增加似乎与全身反应无关。
