Kusakawa You, Yoshida Eiji, Hayakawa Tohru
Department of Dental Engineering, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
Biomed Res Int. 2017;2017:1521593. doi: 10.1155/2017/1521593. Epub 2017 Jan 29.
Protein adsorption onto titanium (Ti) or zirconia (ZrO) was evaluated using a 27 MHz quartz crystal microbalance (QCM). As proteins, fibronectin (Fn), a cell adhesive protein, and albumin (Alb), a cell adhesion-inhibiting protein, were evaluated. The Ti and ZrO sensors for QCM were characterized by atomic force microscopy and electron probe microanalysis observation, measurement of contact angle against water, and surface roughness. The amounts of Fn and Alb adsorbed onto the Ti and ZrO sensors and apparent reaction rate were obtained using QCM measurements. Ti sensor showed greater adsorption of Fn and Alb than the ZrO sensor. In addition, amount of Fn adsorbed onto the Ti or ZrO sensors was higher than that of Alb. The surface roughness and hydrophilicity of Ti or ZrO may influence the adsorption of Fn or Alb. With regard to the adsorption rate, Alb adsorbed more rapidly than Fn onto Ti. Comparing Ti and ZrO, Alb adsorption rate to Ti was faster than that to ZrO. Fn adsorption will be effective for cell activities, but Alb adsorption will not. QCM method could simulate Fn and Alb adsorption to Ti or ZrO.
使用27兆赫兹的石英晶体微天平（QCM）评估蛋白质在钛（Ti）或氧化锆（ZrO）上的吸附情况。作为蛋白质，评估了纤连蛋白（Fn，一种细胞黏附蛋白）和白蛋白（Alb，一种细胞黏附抑制蛋白）。用于QCM的Ti和ZrO传感器通过原子力显微镜、电子探针微分析观察、水接触角测量和表面粗糙度进行表征。使用QCM测量获得吸附在Ti和ZrO传感器上的Fn和Alb的量以及表观反应速率。Ti传感器对Fn和Alb的吸附比ZrO传感器更强。此外，吸附在Ti或ZrO传感器上的Fn的量高于Alb。Ti或ZrO的表面粗糙度和亲水性可能会影响Fn或Alb的吸附。关于吸附速率，Alb在Ti上的吸附比Fn更快。比较Ti和ZrO，Alb在Ti上的吸附速率比在ZrO上更快。Fn吸附对细胞活性有效，但Alb吸附无效。QCM方法可以模拟Fn和Alb在Ti或ZrO上的吸附。
