Fujita Hiroka, Kudo Tada-Aki, Kanetaka Hiroyasu, Miyazaki Toshiki, Hashimoto Masami, Kawashita Masakazu
Graduate School of Biomedical Engineering, Tohoku University, Aoba-ku, Sendai 980-8579, Japan.
Graduate School of Dentistry, Tohoku University, Aoba-ku, Sendai 980-8575, Japan.
ACS Biomater Sci Eng. 2016 Jul 11;2(7):1162-1168. doi: 10.1021/acsbiomaterials.6b00190. Epub 2016 Jun 30.
Artificial hydroxyapatite (HAp) is osteoconductive, but the mechanism is still unclear. It is likely that some serum proteins are adsorbed onto HAp and influence its osteoconductivity. We investigated the adsorption behavior of laminin (LN), which was isolated from murine Engelbreth-Holm-Swarm sarcoma, onto HAp and compared it with nonosteoconductive alpha-type alumina (α-AlO). Cell adhesion, spreading, and proliferation on native and LN-adsorbed discs of HAp or α-AlO were examined using murine MC3T3-E1 osteoblastic cells. A larger amount of LN adsorbed onto HAp than α-AlO despite the electrostatic repulsion between LN and HAp, suggesting the specific adsorption of LN onto HAp. The LN adsorbed onto HAp remarkably enhanced initial attachment and spreading of MC3T3-E1 cells, but subsequent proliferation of MC3T3-E1 cells was influenced by the type of material rather than LN adsorption. These fundamental findings imply that LN adsorbed on HAp could trigger osteoconductivity in vivo, aiding in the development of novel biomaterials that specifically adsorb LN and effectively enhance cell attachment and spreading.
人工合成的羟基磷灰石（HAp）具有骨传导性，但其机制仍不清楚。一些血清蛋白可能吸附在HAp上并影响其骨传导性。我们研究了从小鼠Engelbreth-Holm-Swarm肉瘤中分离出的层粘连蛋白（LN）在HAp上的吸附行为，并将其与无骨传导性的α型氧化铝（α-AlO）进行比较。使用小鼠MC3T3-E1成骨细胞检测了HAp或α-AlO的天然盘和LN吸附盘上的细胞黏附、铺展和增殖情况。尽管LN与HAp之间存在静电排斥，但吸附在HAp上的LN量比α-AlO上的多，这表明LN在HAp上存在特异性吸附。吸附在HAp上的LN显著增强了MC3T3-E1细胞的初始附着和铺展，但MC3T3-E1细胞随后的增殖受材料类型而非LN吸附的影响。这些基础研究结果表明，吸附在HAp上的LN可能在体内引发骨传导性，有助于开发能特异性吸附LN并有效增强细胞附着和铺展的新型生物材料。
