Graduate School of Biomedical Engineering, Tohoku University, Sendai, 980-8579, Japan,
J Mater Sci Mater Med. 2014 Feb;25(2):453-9. doi: 10.1007/s10856-013-5086-z. Epub 2013 Nov 2.
Bone cement containing alumina particles with a specific crystalline structure exhibits the ability to bond with bone. These particles (AL-P) are mainly composed of delta-type alumina (δ-Al2O3). It is likely that some of the proteins present in the body environment are adsorbed onto the cement and influence the expression of its bioactivity. However, the effect that this adsorption of proteins has on the bone-bonding mechanism of bone cement has not yet been elucidated. In this study, we investigated the characteristics of the adsorption of bovine serum albumin (BSA) onto AL-P and compared them with those of its adsorption onto hydroxyapatite (HA), which also exhibits bone-bonding ability, as well as with those of adsorption onto alpha-type alumina (α-Al2O3), which does not bond with bone. The adsorption characteristics of BSA onto AL-P were very different from those onto α-Al2O3 but quite similar to those onto HA. It is speculated that BSA is adsorbed onto AL-P and HA by interionic interactions, while it is adsorbed onto α-Al2O3 by electrostatic attraction. The results suggest that the specific adsorption of albumin onto implant materials might play a role in the expression of the bone-bonding abilities of the materials.
含具有特定晶体结构的氧化铝颗粒的骨水泥表现出与骨结合的能力。这些颗粒（AL-P）主要由 δ 型氧化铝（δ-Al2O3）组成。可能有一些存在于体内环境中的蛋白质被吸附到水泥上，并影响其生物活性的表达。然而，这种蛋白质的吸附对骨水泥骨结合机制的影响尚未阐明。在这项研究中，我们研究了牛血清白蛋白（BSA）在 AL-P 上的吸附特性，并将其与在同样具有骨结合能力的羟基磷灰石（HA）上的吸附特性以及在不与骨结合的 α 型氧化铝（α-Al2O3）上的吸附特性进行了比较。BSA 在 AL-P 上的吸附特性与在 α-Al2O3 上的吸附特性非常不同，但与在 HA 上的吸附特性非常相似。据推测，BSA 通过离子间相互作用吸附到 AL-P 和 HA 上，而通过静电吸引吸附到 α-Al2O3 上。结果表明，白蛋白对植入材料的特异性吸附可能在材料的骨结合能力的表达中起作用。
