Chen Yong Feng, Wu Zhong Min, Xie Cong, Bai Shi, Zhao Li Dong
Department of Basic Medical Sciences, School of Medicine of Taizhou University, Taizhou 318000, China.
ISRN Hematol. 2013 Jun 18;2013:986219. doi: 10.1155/2013/986219. Print 2013.
Parasecretion of the hematopoietic cytokines is considered as one of the mechanisms account for bone marrow hematopoiesis disorder. In this study, the level of IL-6 secreted by bone marrow stromal cells from a mouse model of aplastic anemia was analyzed. The aplastic anemia mouse model was established with cyclophosphamide in combination with chloramphenicol and (60)Co γ radiation. The impairment of bone marrow hematopoiesis induced by irradiation and chemotherapeutic drugs was subsequently characterized by peripheral blood cell count, pathomorphological changes, and apoptosis rate. Furthermore, the in vitro proliferation of bone marrow stromal cells (BMSC) and the IL-6 secretion levels of BMSC were analyzed. In our model of aplastic anemia, the number of peripheral blood cells and bone marrow cells (BMC) were notably decreased, and the apoptosis rate of BMC increased. Furthermore, the proliferation of BMSC was obviously impeded while the IL-6 secretion levels of BMSC significantly increased. The findings of our study suggested that the IL-6 secretion level may be enhanced to some extent by the induction of aplastic anemia caused by irradiation and chemotherapeutic drugs and that the abnormal level of IL-6 might probably interfere with the stability of the bone marrow hematopoietic microenvironment.
造血细胞因子的旁分泌被认为是导致骨髓造血障碍的机制之一。在本研究中，分析了再生障碍性贫血小鼠模型的骨髓基质细胞分泌的白细胞介素-6（IL-6）水平。用环磷酰胺联合氯霉素及（60）Coγ射线建立再生障碍性贫血小鼠模型。随后通过外周血细胞计数、病理形态学变化和凋亡率来表征辐射和化疗药物诱导的骨髓造血损伤。此外，还分析了骨髓基质细胞（BMSC）的体外增殖情况以及BMSC的IL-6分泌水平。在我们的再生障碍性贫血模型中，外周血细胞和骨髓细胞（BMC）数量显著减少，BMC凋亡率增加。此外，BMSC的增殖明显受阻，而BMSC的IL-6分泌水平显著升高。我们的研究结果表明，辐射和化疗药物引起的再生障碍性贫血可能在一定程度上提高IL-6分泌水平，且IL-6水平异常可能会干扰骨髓造血微环境的稳定性。
