Britt B A, Endrenyi L, Cadman D L
Br J Anaesth. 1975 Jun;47(6):650-3. doi: 10.1093/bja/47.6.650.
MHS and normal pigs were anaesthetized with nitrous oxide and diazepam. Halothane 1% was then administered for 1 hr. Immediately before and immediately after the halothane inhalation, 10 g of vastus lateralis muscle was excised. SR was isolated from this muscle. Calcium accumulation by the MHS sarcoplasmic reticulum in the absence of halothane was greater than normal. Halothane in vitro produced a similar increase in calcium uptake into both the MHS and the normal SR. Halothane in vivo significantly reduced calcium accumulation by hyperthermic SR but had no significant effect on calcium uptake into the normal SR. Thus the action of halothane on the MHS sarcoplasmic reticulum is indirect, occurring only in the intact cell. It is possibly a result of the deleterious effect which lack of ATP, a low pH or a high temperature is likely to have on the calcium accumulating mechanism of the SR membrane. Our results suggest that the primary defects of porcine and human malignant hyperthermia are not entirely synonymous.
用氧化亚氮和地西泮对恶性高热易感（MHS）猪和正常猪进行麻醉。然后给予1%的氟烷，持续1小时。在吸入氟烷之前和之后立即切除10克股外侧肌。从该肌肉中分离出肌浆网（SR）。在无氟烷的情况下，MHS肌浆网的钙蓄积量大于正常情况。体外氟烷使MHS和正常肌浆网的钙摄取量有类似增加。体内氟烷显著降低高热肌浆网的钙蓄积，但对正常肌浆网的钙摄取无显著影响。因此，氟烷对MHS肌浆网的作用是间接的，仅在完整细胞中发生。这可能是由于缺乏三磷酸腺苷（ATP）、低pH值或高温可能对肌浆网膜的钙蓄积机制产生的有害作用所致。我们的结果表明，猪和人类恶性高热的主要缺陷并不完全相同。
