Gronert G A, Heffron J J, Taylor S R
Eur J Pharmacol. 1979 Sep 15;58(2):179-87. doi: 10.1016/0014-2999(79)90010-4.
To examine the function of sarcoplasmic reticulum (SR) in malignant hyperthermia, SR was isolated from semitendinosus muscle of normal and genetically susceptible Poland China swine. Determinations included rate of calcium binding (oxalate absent), rate and capacity of calcium uptake (oxalate present), and spontaneous calcium release (in the absence of ionic depolarization or calcium) with and without halothane, using the millipore filtration technique. Rate of calcium binding, and rate and capacity of calcium uptake were decreased, and spontaneous calcium release was greater in SR fragments from susceptible swine as compared to those from normal swine. Halothane 0.5% slightly increased the rate of calcium binding in susceptible and normal SR. Above 1%, halothane decreased calcium binding rate, and uptake rate and capacity, and increased calcium release similarly in susceptible and normal SR. These differences in SR function were insufficient to explain the etiology of malignant hyperthemia, nor did the effect of halothane account for its triggering action.
为研究肌浆网（SR）在恶性高热中的作用，从正常及遗传易感性波兰中国猪的半腱肌中分离出肌浆网。测定内容包括钙结合速率（无草酸盐）、钙摄取速率和容量（有草酸盐），以及在有无氟烷情况下，利用微孔过滤技术测定自发钙释放（无离子去极化或钙时）。与正常猪的肌浆网片段相比，易感性猪的肌浆网片段中钙结合速率、钙摄取速率和容量降低，自发钙释放增加。0.5%的氟烷使易感性和正常肌浆网中的钙结合速率略有增加。高于1%时，氟烷降低钙结合速率、摄取速率和容量，并使易感性和正常肌浆网中的钙释放同样增加。肌浆网功能的这些差异不足以解释恶性高热的病因，氟烷的作用也不能说明其触发作用。
