Castro Bruno Gomes de, Souza Miliane Moreira Soares de, Regua-Mangia Adriana Hamond, Bittencourt Avelino José
Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso, Sinop, MT, Brazil.
Rev Bras Parasitol Vet. 2013 Apr-Jun;22(2):318-21. doi: 10.1590/S1984-29612013000200052.
This study aimed to verify the occurrence of Shiga toxin-producing Escherichia coli (STEC) strains in three distinct anatomic parts of the stable fly Stomoxys calcitrans by multiplex polymerase chain reaction (PCR Multiplex). According to the results obtained, E. coli was identified in 19.5% of the stable flies. Shiga toxin genes were detected in 13% of the E. coli isolated, most frequently from the surface, followed by abdominal digestive tract and mouth apparatus of insects, respectively. This is the first study to detect presence of STEC in Stomoxys calcitrans in Brazil; it has also revealed the potential role of stable flies as carriers of pathogenic bacterial agents.
本研究旨在通过多重聚合酶链反应（多重PCR）验证在厩螫蝇Stomoxys calcitrans三个不同解剖部位中产生志贺毒素的大肠杆菌（STEC）菌株的存在情况。根据所得结果，在19.5%的厩螫蝇中鉴定出了大肠杆菌。在分离出的大肠杆菌中，13%检测到了志贺毒素基因，最常见于昆虫的体表，其次分别是腹部消化道和口器。这是巴西首次检测到厩螫蝇中存在STEC的研究；该研究还揭示了厩螫蝇作为致病细菌载体的潜在作用。
