Department of Biochemistry & Molecular Biology, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
Exp Biol Med (Maywood). 2013 May;238(5):509-18. doi: 10.1177/1535370213488474.
In this minireview, we focus on advances in our knowledge of the human erythrocyte proteome and interactome that have occurred since our seminal review on the topic published in 2007. As will be explained, the number of unique proteins has grown from 751 in 2007 to 2289 as of today. We describe how proteomics and interactomics tools have been used to probe critical protein changes in disorders impacting the blood. The primary example used is the work done on sickle cell disease where biomarkers of severity have been identified, protein changes in the erythrocyte membranes identified, pharmacoproteomic impact of hydroxyurea studied and interactomics used to identify erythrocyte protein changes that are predicted to have the greatest impact on protein interaction networks.
在这篇简评中，我们重点介绍了自 2007 年发表关于该主题的开创性综述以来，人类红细胞蛋白质组学和相互作用组学知识的进展。正如将要解释的那样，独特蛋白质的数量从 2007 年的 751 个增加到了今天的 2289 个。我们描述了蛋白质组学和相互作用组学工具如何用于探测影响血液的疾病中的关键蛋白质变化。主要例子是在镰状细胞病方面的工作，其中已经确定了严重程度的生物标志物，鉴定了红细胞膜中的蛋白质变化，研究了羟基脲的药物蛋白质组学影响，并利用相互作用组学来鉴定预计对蛋白质相互作用网络有最大影响的红细胞蛋白质变化。
