Tantra Ratna, Robinson Kenneth, Sikora Aneta
Surface and Nanoanalysis, National Physical Laboratory, Teddington, UK.
Electrophoresis. 2014 Feb;35(2-3):263-70. doi: 10.1002/elps.201300175. Epub 2013 Sep 19.
Microfluidic CE with conductivity detection platforms could have an impact on the future development of smaller, faster and portable devices. However, for the purpose of reliable identification and quantification, there is a need to understand the degree of irreproducibility associated with the analytical technique. In this study, a protocol was developed to remove baseline drift problems sometimes observed in such devices. The protocol, which consisted of pre-conditioning steps prior to analysis, was used to further assess measurement variability from 24 individual microchips fabricated from six separate batches of glass substrate. Results show acceptable RSD percentage for retention time measurements but large variability in their corresponding peak areas (with some microchips having variability of ∼50%). Sources of variability were not related to substrate batch but possibly to a number of factors such as applied voltage fluctuations or variations in microchannel quality, for example surface roughness that will subsequently affect microchannel dimensions.
具有电导率检测平台的微流控毛细管电泳可能会对更小、更快、更便携设备的未来发展产生影响。然而，为了进行可靠的识别和定量，有必要了解与该分析技术相关的不可重复性程度。在本研究中，开发了一种方案来消除此类设备中有时观察到的基线漂移问题。该方案包括分析前的预处理步骤，用于进一步评估由六批不同玻璃基板制造的24个单独微芯片的测量变异性。结果表明，保留时间测量的相对标准偏差（RSD）百分比可接受，但其相应峰面积的变异性较大（一些微芯片的变异性约为50%）。变异性来源与基板批次无关，但可能与一些因素有关，例如施加电压波动或微通道质量变化，例如表面粗糙度，这随后会影响微通道尺寸。
