Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Bachelor program of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4469-4476. doi: 10.1099/ijs.0.052837-0. Epub 2013 Jul 16.
Two Gram-staining-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated and non-spore-forming amylolytic marine bacterial strains, designated CC-AMZ-30M(T) and CC-AMZ-30N(T), were isolated from coastal surface seawater in Taiwan. Strain CC-AMZ-30M(T) shared pairwise 16S rRNA gene sequence similarities of 95.8, 95.0 and <94.0 % to Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. Strain CC-AMZ-30N(T) shared 97.0, 96.7, 95.0 and <95.1 % similarities to strain CC-AMZ-30M(T), Sphingomicrobium lutaoense CC-TBT-3(T), Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) and other sphingomonads, respectively. The common polar lipids of the two strains include a signature glycolipid (GL2), diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingoglycolipid in major amounts besides moderate-to-trace amounts of an unidentified aminolipid and several unidentified glycolipids. Both strains contained C18 : 1ω7c/C18 : 1ω6c, C16 : 1ω7c/C16 : 1ω6c, C16 : 0 and C18 : 1 2-OH as major (>5 % of the total) fatty acids. Strains CC-AMZ-30M(T) and CC-AMZ-30N(T) had DNA G+C contents of 64.2 and 65.2 mol%, respectively. The major polyamine was spermidine in strain CC-AMZ-30M(T) and triamine sym-homospermidine in strain CC-AMZ-30N(T). Both strains contained ubiquinone Q-10 as the predominant respiratory quinone. Differential phylogenetic and chemotaxonomic evidence including the presence of characteristic GL2, C18 : 1 2-OH and several other phenotypic features supported the classification of strains CC-AMZ-30M(T) and CC-AMZ-30N(T) as two novel species of the genus Sphingomicrobium, for which we propose the names Sphingomicrobium marinum sp. nov. and Sphingomicrobium flavum sp. nov., respectively; corresponding type strains are Sphingomicrobium marinum CC-AMZ-30M(T) ( = JCM 18554(T) = BCRC 80466(T)) and Sphingomicrobium flavum CC-AMZ-30N(T) ( = JCM 18555(T) = BCRC 80467(T)). An emended description of the genus Sphingomicrobium is also proposed.
两株革兰氏阴性、黄色、杆状、严格需氧、无鞭毛、不产芽孢的海洋淀粉分解细菌菌株，命名为 CC-AMZ-30M(T) 和 CC-AMZ-30N(T)，从台湾沿海表面海水中分离得到。菌株 CC-AMZ-30M(T) 与 Sphingomicrobium lutaoense CC-TBT-3(T)、Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) 和其他鞘氨醇单胞菌的 16S rRNA 基因序列相似性分别为 95.8%、95.0%和 <94.0%。菌株 CC-AMZ-30N(T) 与菌株 CC-AMZ-30M(T)、Sphingomicrobium lutaoense CC-TBT-3(T)、Sphingomicrobium astaxanthinifaciens CC-AMO-30B(T) 和其他鞘氨醇单胞菌的相似性分别为 97.0%、96.7%、95.0%和 <95.1%。这两种菌株的共同极性脂包括特征性糖脂 (GL2)、双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和神经酰胺糖脂，此外还有中等至微量的未鉴定的胺脂和几种未鉴定的糖脂。两种菌株均含有 C18:1ω7c/C18:1ω6c、C16:1ω7c/C16:1ω6c、C16:0 和 C18:1 2-OH 作为主要 (>5%总脂肪酸)脂肪酸。菌株 CC-AMZ-30M(T) 和 CC-AMZ-30N(T) 的 DNA G+C 含量分别为 64.2mol%和 65.2mol%。菌株 CC-AMZ-30M(T) 中的主要多胺是亚精胺，而菌株 CC-AMZ-30N(T) 中的主要多胺是三胺对称同亚精胺。两种菌株均含有泛醌 Q-10 作为主要的呼吸醌。基于特征性 GL2、C18:1 2-OH 和其他几种表型特征的差异系统发育和化学分类学证据支持菌株 CC-AMZ-30M(T) 和 CC-AMZ-30N(T) 分类为鞘氨醇单胞菌属的两个新种，分别命名为海洋鞘氨醇单胞菌 Sphingomicrobium marinum sp. nov. 和黄鞘氨醇单胞菌 Sphingomicrobium flavum sp. nov.；相应的模式菌株为海洋鞘氨醇单胞菌 CC-AMZ-30M(T) ( = JCM 18554(T) = BCRC 80466(T)) 和黄鞘氨醇单胞菌 CC-AMZ-30N(T) ( = JCM 18555(T) = BCRC 80467(T))。还提出了鞘氨醇单胞菌属的修订描述。
