Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Bachelor program of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4533-4539. doi: 10.1099/ijs.0.053553-0. Epub 2013 Aug 1.
A novel, Gram-staining-negative, non-flagellated, oval or short-rod-shaped, strictly aerobic and non-spore-forming marine bacterium, designated strain CC-AMW-C(T), was isolated from coastal surface seawater in Kending County, Taiwan. Cells of strain CC-AMW-C(T) displayed unusual morphology and formed colourless or beige colonies on marine agar. The isolate shared pairwise 16S rRNA gene sequence similarities of 97.2 and 97.1 % with Shimia marina BCRC 80068(T) and Shimia isoporae BCRC 80085(T), respectively, and established a discrete phyletic lineage closely associated with the members of the genus Shimia. DNA-DNA hybridization values indicated <18.2 % genomic relatedness with species of the genus Shimia. The polar lipid profile of strain CC-AMW-C(T) comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, four unidentified lipids and an unidentified phospholipid. The predominant fatty acids were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8; 75.5 %). The DNA G+C content was 61.2 mol%. The predominant respiratory quinone was ubiquinone Q-10 and the major polyamine was cadaverine. The chemotaxonomic evidence, including extraordinary amounts of C18 : 1ω7c and/or C18 : 1ω6c, major polar lipids, polyamine, quinone and DNA G+C contents of CC-AMW-C(T), was in line with that of the members of the genus Shimia. Thus, strain CC-AMW-C(T) should be classified as a novel species of the genus Shimia, for which the name Shimia biformata sp. nov. is proposed. The type strain is CC-AMW-C(T) ( = JCM 18818(T) = BCRC 80548(T)). An emended description of the genus Shimia is also proposed.
一株新型革兰氏阴性、无鞭毛、椭圆形或短杆形、严格需氧、非孢子形成的海洋细菌，被命名为 CC-AMW-C(T) 菌株，从台湾垦丁县沿海表层海水中分离得到。CC-AMW-C(T) 菌株的细胞形态异常，在海洋琼脂上形成无色或米色菌落。该分离株与 Shimia marina BCRC 80068(T) 和 Shimia isoporae BCRC 80085(T) 的 16S rRNA 基因序列相似性分别为 97.2%和 97.1%，并与 Shimia 属的成员形成一个离散的系统发育谱系。DNA-DNA 杂交值表明与 Shimia 属的种之间的基因组相关性<18.2%。CC-AMW-C(T) 菌株的极性脂谱由二磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、两种未鉴定的胺脂、四种未鉴定的脂和一种未鉴定的磷脂组成。主要脂肪酸为 C18:1ω7c 和/或 C18:1ω6c（特征 8 总和；75.5%）。DNA G+C 含量为 61.2mol%。主要呼吸醌为泛醌 Q-10，主要多胺为尸胺。包括 C18:1ω7c 和/或 C18:1ω6c、主要极性脂、多胺、醌和 DNA G+C 含量在内的化学生物分类学证据，与 Shimia 属的成员一致。因此，CC-AMW-C(T) 菌株应归类为 Shimia 属的一个新型种，命名为 Shimia biformata sp. nov.。模式菌株为 CC-AMW-C(T)（=JCM 18818(T)=BCRC 80548(T)）。还提出了 Shimia 属的修订描述。
