Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117312 Moscow, Russia.
Center 'Bioengineering', Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/1, 117312 Moscow, Russia.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4456-4461. doi: 10.1099/ijs.0.054536-0. Epub 2013 Jul 16.
An obligately alkaliphilic, anaerobic, thermo- and halotolerant, spore-forming bacterium was isolated from sediments of soda lake Magadi (Kenya) and designated strain Z-1001(T). Cells of strain Z-1001(T) were straight, Gram-positive rods, slowly motile. Strain Z-1001(T) was found to be an obligate anaerobe. It grew within a pH range from 7.5 to 10.7 with an optimum at 9.25-9.5 (at 40 °C), a temperature range from 20 to 57 °C with an optimum at 45-50 °C, and a NaCl concentration range from 0 to 1.55 M with an optimum at 1.2-1.4 M. Peptides, such as meat and yeast extracts, peptone and tryptone, were fermented by Z-1001(T). Carbohydrates did not support growth. With yeast extract as an electron donor, strain Z-1001(T) reduced S(2)O(3)(2-), NO(-)(3), AsO(3-)(4), Fe(III) citrate and anthraquinone-2,6-disulfonate (AQDS) as electron acceptors. The isolate was able to grow oligotrophically with a very small amount of yeast extract: 0.03 g l(-1). The main fatty acids were C16 : 0, C16 : 1ω7c, C18 : 0 and C18 : 1ω9. The DNA G+C content of the isolate was 35.6 mol%. 16S rRNA gene sequence analysis showed that strain Z-1001(T) is a member of family Natranaerobiaceae, clustering with the type strain of Natranaerobius thermophilus (95.8-96.0 % sequence similarity). On the basis of physiological and phylogenetic data it is proposed that strain Z-1001(T) ( = DSM 24923(T) = VKM B-2666(T)) represents a novel genus and species, Natranaerobaculum magadiense gen. nov., sp. nov.
一株严格的嗜堿、厌氧、耐热和嗜盐、产芽孢的细菌，从肯尼亚马加迪（Magadi）苏打湖的沉积物中分离得到，并被命名为 Z-1001(T) 菌株。Z-1001(T) 菌株的细胞为直的、革兰氏阳性棒状，缓慢运动。Z-1001(T) 菌株被发现是一种专性厌氧菌。它在 pH 值为 7.5 至 10.7 的范围内生长，最适 pH 值为 9.25-9.5（在 40°C 时），温度范围为 20 至 57°C，最适温度为 45-50°C，NaCl 浓度范围为 0 至 1.55M，最适浓度为 1.2-1.4M。Z-1001(T) 可以发酵多肽，如肉和酵母提取物、蛋白胨和色氨酸。碳水化合物不能支持生长。当以酵母提取物作为电子供体时，Z-1001(T) 菌株可以将 S(2)O(3)(2-)、NO(-)(3)、AsO(3-)(4)、Fe(III) 柠檬酸盐和蒽醌-2,6-二磺酸盐（AQDS）还原为电子受体。该分离株可以在非常少量的酵母提取物（0.03 g l(-1)）的条件下进行寡营养生长。主要脂肪酸为 C16 : 0、C16 : 1ω7c、C18 : 0 和 C18 : 1ω9。该分离株的 DNA G+C 含量为 35.6 mol%。16S rRNA 基因序列分析表明，Z-1001(T) 菌株是 Natranaerobiaceae 科的成员，与嗜热 Natranaerobius thermophilus 的模式株（95.8-96.0%序列相似性）聚类。基于生理和系统发育数据，建议 Z-1001(T) 菌株（=DSM 24923(T)=VKM B-2666(T)）代表一个新的属和种，即 Magadi 嗜堿厌氧菌属，新属，新种。
