Winogradsky Institute of Microbiology of Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, 60-let Oktyabrya prospect 7/2, 117312, Moscow, Russia.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4730-4738. doi: 10.1099/ijsem.0.004341. Epub 2020 Jul 22.
An obligately alkaliphilic, anaerobic, proteolytic bacterium was isolated from a sample of Tanatar III soda lake sediment (Altai region, Russia) and designated as strain Z-1701. Cells of strain Z-1701 were short, straight, motile Gram-stain-positive rods. Growth of Z-1701 obligately depended on the presence of sodium carbonate. Strain Z-1701 could utilize various peptides mixtures, such as beef and yeast extracts, peptone, soytone, trypticase and tryptone, as well as such proteins as albumin, gelatin and sodium caseinate. It was able to grow oligotrophically with 0.02 g l yeast extract as the sole energy and carbon source. Carbohydrates did not support the growth of strain Z-1701. The main products released during the growth of strain Z-1701 on tryptone were formate, acetate and ammonium. Strain Z-1701 was able to reduce ferrihydrite, Fe(III)-EDTA, anthraquinone-2,6-disulfonate and elemental sulfur, using proteinaceous substrates as electron donors. In all cases the presence of the electron acceptor in the medium stimulated growth. The main cellular fatty acids were iso-C, iso-C aldehyde, iso-C ω6, C, iso-C aldehyde, C aldehyde and C. The DNA G+C content of the isolate was 43.9 mol%. Phylogenetic analysis based on the concatenated alignment of 120 protein-marker sequences revealed that strain Z-1701 falls into a cluster with the genus , family . 16S rRNA gene sequence identity between strain Z-1701 and species were 88.3-89.75 %. On the basis of its phenotypic characteristics and phylogenetic position, the novel isolate is considered to be a representative of a novel genus and species for which the name gen. nov., sp. nov. is proposed, with Z-1701 (=JCM 32929=DSM 109060=VKM B-3261) as its type strain.
一株严格嗜碱、厌氧、蛋白水解的细菌从塔那特 III 苏打湖沉积物（俄罗斯阿尔泰地区）样本中分离出来，并被命名为 Z-1701 株。Z-1701 株的细胞为短而直的运动性革兰氏阳性棒状。Z-1701 株的生长严格依赖于碳酸钠的存在。Z-1701 株可以利用各种肽混合物，如牛肉和酵母提取物、蛋白胨、大豆胨、胰蛋白酶和胰蛋白胨，以及白蛋白、明胶和酪蛋白酸钠等蛋白质作为唯一的能源和碳源。它可以在含有 0.02g/L 酵母提取物的贫营养条件下生长。碳水化合物不能支持 Z-1701 株的生长。Z-1701 株在胰蛋白胨上生长时释放的主要产物是甲酸盐、乙酸盐和铵盐。Z-1701 株可以利用蛋白质作为电子供体还原水铁矿、Fe(III)-EDTA、蒽醌-2,6-二磺酸钠和元素硫。在所有情况下，培养基中电子受体的存在都能刺激生长。主要的细胞脂肪酸是 iso-C、iso-C 醛、iso-Cω6、C、iso-C 醛、C 醛和 C。该分离株的 DNA G+C 含量为 43.9mol%。基于 120 个蛋白标记序列的串联比对的系统发育分析表明，Z-1701 株属于 属， 科。Z-1701 株与 种的 16S rRNA 基因序列同源性为 88.3-89.75%。基于其表型特征和系统发育位置，该新分离株被认为是一个新属和新种的代表，建议采用新属名 gen. nov.，新种名 sp. nov.，其模式菌株为 Z-1701 株（=JCM 32929=DSM 109060=VKM B-3261）。
