Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, Fujian Province 361021, China.
Bioresour Technol. 2013 Sep;144:74-9. doi: 10.1016/j.biortech.2013.06.093. Epub 2013 Jun 29.
Microbes play irreplaceable role in oxygen reduction reaction of biocathode in microbial fuel cells (MFCs). In this study, air-diffusion biocathode MFCs were set up for accelerating oxygen reduction and microbial community analysis. Linear sweep voltammetry and Tafel curve confirmed the function of cathode biofilm to catalyze oxygen reduction. Microbial community analysis revealed higher diversity and richness of community in plankton than in biofilm. Proteobacteria was the shared predominant phylum in both biofilm and plankton (39.9% and 49.8%) followed by Planctomycetes (29.9%) and Bacteroidetes (13.3%) in biofilm, while Bacteroidetes (28.2%) in plankton. Minor fraction (534, 16.4%) of the total operational taxonomic units (3252) was overlapped demonstrating the disproportionation of bacterial distribution in biofilm and plankton. Pseudomonadales, Rhizobiales and Sphingobacteriales were exoelectrogenic orders in the present study. The research obtained deep insight of microbial community and provided more comprehensive information on uncultured rare bacteria.
微生物在微生物燃料电池（MFC）的生物阴极的氧还原反应中发挥着不可替代的作用。在这项研究中，我们构建了空气扩散生物阴极 MFC，以加速氧气还原和微生物群落分析。线性扫描伏安法和塔菲尔曲线证实了阴极生物膜催化氧气还原的功能。微生物群落分析显示，浮游生物中的群落多样性和丰富度高于生物膜。在生物膜和浮游生物中，变形菌门都是共同的主要门（分别为 39.9%和 49.8%），其次是浮霉菌门（29.9%）和拟杆菌门（13.3%），而在浮游生物中，拟杆菌门（28.2%）。总操作分类单元（3252）中只有一小部分（534，16.4%）重叠，表明生物膜和浮游生物中细菌分布的不均匀性。假单胞菌目、根瘤菌目和鞘脂单胞菌目是本研究中的放电子目。该研究深入了解了微生物群落，并提供了更多关于未培养稀有细菌的综合信息。
