el-Naggar A K, Batsakis J G, Teague K, Giacco G, Guinee V F, Swanson D
Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77036.
Am J Pathol. 1990 Aug;137(2):275-80.
To explore the potential role that ribonucleic acid (RNA) content analysis may have in the assessment of primary renal cell carcinomas (RCC), biparametric flow cytometric (acridine orange) measurements for DNA/RNA were obtained on 108 fresh neoplastic specimens. RNA content was divided into low and high groups, based on the average RNA content in normal kidney controls. High RNA content was significantly correlated with aneuploidy, high proliferative index, high nuclear grade, cytoplasmic granularity, and large tumor size. No correlation was found between RNA content and patients' sex, race, and clinical stage of the carcinomas. When diploid RCCs were separately analyzed, high RNA content was correlated with high nuclear grade, large tumor size, high clinical stage, and cytoplasmic granularity. There was no correlation between RNA content and the patient's sex or race or the neoplasm's proliferative index. Of the 16 patients that relapsed (5 diploid and 11 aneuploid), four of the diploid and all 11 aneuploid neoplasms displayed high RNA content. The authors' data show that RNA may be a valuable objective and quantitative parameter in the clinicopathologic assessment of RCC.
为了探究核糖核酸（RNA）含量分析在原发性肾细胞癌（RCC）评估中可能具有的潜在作用，对108份新鲜肿瘤标本进行了DNA/RNA的双参数流式细胞术（吖啶橙）测量。根据正常肾对照中的平均RNA含量，将RNA含量分为低组和高组。高RNA含量与非整倍体、高增殖指数、高核分级、细胞质颗粒度以及大肿瘤尺寸显著相关。未发现RNA含量与患者的性别、种族以及癌的临床分期之间存在相关性。当对二倍体RCC进行单独分析时，高RNA含量与高核分级、大肿瘤尺寸、高临床分期以及细胞质颗粒度相关。RNA含量与患者的性别、种族或肿瘤的增殖指数之间无相关性。在16例复发患者（5例二倍体和11例非整倍体）中，4例二倍体和所有11例非整倍体肿瘤均显示高RNA含量。作者的数据表明，RNA可能是RCC临床病理评估中有价值的客观定量参数。
