MacNeill Christopher M, Wailes Elizabeth M, Levi-Polyachenko Nicole H
Department of Plastic and Reconstructive Surgery, Wake Forest University Baptist Medical Center, 100 Medical Center Blvd. Winston-Salem, NC 27157, USA.
J Nanosci Nanotechnol. 2013 Jun;13(6):3784-91. doi: 10.1166/jnn.2013.7211.
The photothermal efficiency of two similar organic nanomaterials, poly(3,4-ethylenedioxythiophene):poly(4-styrene-sulfonate) (PEDOT:PSS) nanoparticles and poly(3,4-ethylenedioxythiophene) (PEDOT) nanotubes, are compared. The PEDOT:PSS nanoparticles ranged from 100-200 nm in diameter, while the PEDOT nanotubes ranged from 200-400 nm in diameter and 4-10 microm in length. By changing the aspect ratio of the PEDOT nanomaterials from a spherical to a tubular shape, interesting differences in the optical and electronic properties of the materials were realized. Because of this, the PEDOT nanotubes were shown to generate on average approximately to 10 degrees C better internal heating for similar concentrations compared to the PEDOT:PSS nanoparticles. Cytotoxicity studies of both nanomaterials showed no significant toxicity towards RKO or HCT116 colorectal cancer cells in the absence of NIR light. The NIR-mediated photothermal efficiency of the PEDOT:PSS nanoparticles and the PEDOT nanotubes were compared in-vitro. A cell viability assay was performed and at the highest concentration (0.1 mg/mL) of nanomaterial, cell survival was close to 20% for the PEDOT:PSS nanoparticles with both RKO and HCT116 cells. Consequently, cell survival for the PEDOT nanotubes was less than 5% for both RKO and HCT116 cells. An in-vitro three dimensional tumor model was assembled using collagen gel tissue phantoms. The depth of heat penetration from the PEDOT nanotubes into the tissue phantoms, along with cell viability of RKO and HCT116 cells was determined and quantified.
比较了两种类似的有机纳米材料——聚（3,4 - 亚乙基二氧噻吩）：聚（4 - 苯乙烯磺酸盐）（PEDOT:PSS）纳米颗粒和聚（3,4 - 亚乙基二氧噻吩）（PEDOT）纳米管的光热效率。PEDOT:PSS纳米颗粒的直径范围为100 - 200纳米，而PEDOT纳米管的直径范围为200 - 400纳米，长度为4 - 10微米。通过将PEDOT纳米材料的纵横比从球形改变为管状，实现了材料光学和电子性质的有趣差异。因此，与PEDOT:PSS纳米颗粒相比，在相似浓度下，PEDOT纳米管平均显示出约10摄氏度更好的内部加热效果。两种纳米材料的细胞毒性研究表明，在没有近红外光的情况下，对RKO或HCT116结肠癌细胞没有显著毒性。在体外比较了PEDOT:PSS纳米颗粒和PEDOT纳米管的近红外介导光热效率。进行了细胞活力测定，在纳米材料的最高浓度（0.1毫克/毫升）下，对于RKO和HCT116细胞，PEDOT:PSS纳米颗粒的细胞存活率接近20%。因此，对于RKO和HCT116细胞，PEDOT纳米管的细胞存活率均小于5%。使用胶原凝胶组织模型组装了体外三维肿瘤模型。确定并量化了PEDOT纳米管进入组织模型的热穿透深度以及RKO和HCT116细胞的细胞活力。
