Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Campus de la Plaine, CP 221, Boulevard du Triomphe, 1050 Brussels, Belgium.
Water Res. 2013 Sep 15;47(14):4938-47. doi: 10.1016/j.watres.2013.05.036. Epub 2013 Jun 13.
In the present study, the antimicrobial resistant (AR) bacteria were quantified and identified in different river samples using in parallel a culture-based approach and a culture-independent one. The objective was to evaluate the importance of the cultivation bias when studying antimicrobial resistance among environmental bacteria. Three different river samples covering a gradient of anthropic influence were tested and three different antimicrobial compounds were used as selective agents: amoxicillin, tetracycline and sulfamethoxazole. From a quantitative point of view, our results highlight the importance of the culture media used, as for the same sample and the same selective agent significant differences were observed in the counts of culturable AR bacteria depending on the culture media used. The identification of AR bacteria through culture or culture-independent methods put on evidence AR bacterial communities that differ dramatically: γ-proteobacteria and more specifically Aeromonadaceae dominated among the isolates while β-proteobacteria (Comamonadaceae), dominated among the sequences obtained without culture. Altogether these results highlight the necessity to develop a methodological consensus preferably without culture, to approach this important topic in the coming years.
在本研究中，我们使用基于培养的方法和非培养的方法平行地对不同河流样本中的抗菌耐药（AR）细菌进行定量和鉴定。目的是评估在研究环境细菌中的抗菌耐药性时，培养偏见的重要性。我们测试了三种不同的河流样本，覆盖了人为影响的梯度，并使用三种不同的抗菌化合物作为选择性试剂：阿莫西林、四环素和磺胺甲恶唑。从定量的角度来看，我们的结果强调了所用培养基的重要性，因为对于相同的样本和相同的选择性试剂，根据所用的培养基，可培养的 AR 细菌的数量存在显著差异。通过培养或非培养方法对 AR 细菌的鉴定表明，AR 细菌群落存在显著差异：在分离株中，γ-变形菌（尤其是气单胞菌科）占主导地位，而在无培养条件下获得的序列中，β-变形菌（丛毛单胞菌科）占主导地位。总之，这些结果强调需要制定一个优选无培养的方法学共识，以便在未来几年中解决这个重要的课题。
