Laboratoire de Bactériologie, CHU SUD d'Amiens, Centre de Biologie Humaine, Salouël, Amiens, France.
J Clin Microbiol. 2013 Oct;51(10):3183-91. doi: 10.1128/JCM.00860-13. Epub 2013 Jul 17.
In a series of 82 Staphylococcus strains isolated from culture, 100% were identified as Staphylococcus aureus by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS); 99.9% (77/82) of them were resistant to benzylpenicillin, oxacillin, and cefoxitin, and 6.1% (5/82) were susceptible to methicillin. Xpert MRSA/SA assay results were concordant with the phenotypic results in 76.8% (63/82) of cases and discordant in 23.2% (19/82) of cases. The MRSA/SA ELITe MGB kit results were concordant with phenotypic results in 100% of the cases. When comparing the Xpert MRSA/SA assay results with the MRSA/SA ELITe MGB kit results, 78% (64/82) of the cases were concordant, while 22% (18/82) of the cases were discordant. No statistically significant differences were observed between the two techniques. The PCR protocol that was used to validate the results of these two methods gave the following results: 49 were conventional methicillin-resistant S. aureus (MRSA) isolates (mecA positive and mecALGA251 negative), and 25 were phenotypic MRSA isolates (mecA negative and mecALGA251 positive).
在一系列从培养物中分离的 82 株葡萄球菌菌株中，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定 100%为金黄色葡萄球菌；99.9%（77/82）对苯唑西林、苯唑西林和头孢西丁耐药，6.1%（5/82）对甲氧西林敏感。Xpert MRSA/SA 检测结果与 76.8%（63/82）的表型结果一致，23.2%（19/82）的结果不一致。MRSA/SA ELITe MGB 试剂盒结果与表型结果 100%一致。当比较 Xpert MRSA/SA 检测结果与 MRSA/SA ELITe MGB 试剂盒结果时，78%（64/82）的病例一致，22%（18/82）的病例不一致。两种技术之间无统计学差异。用于验证这两种方法结果的 PCR 方案得到以下结果：49 株为常规耐甲氧西林金黄色葡萄球菌（MRSA）分离株（mecA 阳性和 mecALGA251 阴性），25 株为表型 MRSA 分离株（mecA 阴性和 mecALGA251 阳性）。
