Wernigerode Branch, Robert Koch Institute, Wernigerode, Germany.
PLoS One. 2011;6(9):e24360. doi: 10.1371/journal.pone.0024360. Epub 2011 Sep 8.
MRSA CC130 containing the mecA homologue mecA(LGA251) were reported from the UK and from Denmark so far from cattle and humans. Here we report on 11 MRSA CC130 among a sample of 12691 isolates of human origin collected from January 2006 until June 2011. MRSA CC130 grew insufficiently on chromogernic agar plates for detection of MRSA; the agglutination test for presence of PBP2a was negative. We designed primers for specific detection of mecA(LGA251) as well as for concomitant detection of both, mec(LGA251) and mecA. As already described, the isolates exhibited spa-types t843, t1736, and t1773. The ccrA homologue indicated the presence SCCmec(XI). When subjected to further characterization by means of a commercially available microarray the isolates were negative for sak chp, and scn, and as expected positive for hla, untruncated hlb, and hld. They furthermore contained edinB, aur, slpA, slpB, slpE. From genes coding for surface and cell wall associated products the ica-operon, cap8, clfA, clfF, ebpS, fnbA, fnbB, sdrC were detected but not cna. The isolates were negative for enterotoxin genes and tst, as well as for eta, and etb; agr-type was III.
迄今为止，英国和丹麦已从牛和人身上分离出携带 mecA 同源物 mecA(LGA251)的耐甲氧西林金黄色葡萄球菌（MRSA）CC130。在此，我们报告了从 2006 年 1 月至 2011 年 6 月期间采集的 12691 例人源分离株中发现的 11 株 MRSA CC130。MRSA CC130 在显色琼脂平板上生长不良，无法检测到 MRSA；针对 PBP2a 存在的凝集试验呈阴性。我们设计了用于特异性检测 mecA(LGA251)以及同时检测 mecA(LGA251)和 mecA 的引物。如前所述，这些分离株表现出 spa 型 t843、t1736 和 t1773。ccrA 同源物表明存在 SCCmec(XI)。当通过商业上可用的微阵列进行进一步鉴定时，这些分离株对 sak chp 和 scn 呈阴性，对 hla、未截断的 hlb 和 hld 呈阳性，如预期的那样。它们还含有 edinB、aur、slpA、slpB、slpE。从编码表面和细胞壁相关产物的基因中，检测到了 ica 操纵子、cap8、clfA、clfF、ebpS、fnbA、fnbB、sdrC，但未检测到 cna。这些分离株对肠毒素基因 tst 以及 eta 和 etb 呈阴性；agr 型为 III 型。
