环磷酰胺治疗百草枯诱导的大鼠肺损伤的剂量。
The dose of cyclophosphamide for treating paraquat-induced rat lung injury.
机构信息
Department of Internal Medicine, Soonchunhyang University Cheonan Hospital, Cheonan, Korea.
出版信息
Korean J Intern Med. 2013 Jul;28(4):420-7. doi: 10.3904/kjim.2013.28.4.420. Epub 2013 Jul 1.
BACKGROUND/AIMS: Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury.
METHODS
Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded.
RESULTS
The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-β1 level.
CONCLUSIONS
A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-β1 levels.
背景/目的：环磷酰胺（CP）是治疗百草枯（PQ）中毒严重病例的一种有前途的治疗方法。我们研究了 CP 的有效剂量，以减轻 PQ 引起的肺损伤。
方法
成年雄性 Sprague-Dawley 大鼠分为五组：对照组、PQ（35mg/kg，腹腔注射）和 PQ+CP（1.5、15 或 30mg/kg）。使用 X 射线微断层扫描（micro-CT）确定肺损伤的程度，并记录组织学变化和细胞因子水平。
结果
micro-CT 结果表明，CP 15mg/kg 比 CP 1.5mg/kg 更有效地治疗 PQ 引起的肺损伤。CP 1.5mg/kg 可减轻炎症的组织学证据，并改变超氧化物歧化酶活性。使用 15mg/kg CP 可降低升高的过氧化氢酶活性和血清转化生长因子（TGF）-β1 水平。
结论
CP 剂量>15mg/kg 可有效降低组织学和 micro-CT 组织检查确定的 PQ 诱导的肺损伤严重程度，可能通过调节抗氧化酶和 TGF-β1 水平。
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