Li Ang, Liu Yuguang, Zhai Lu, Wang Liying, Lin Zhe, Wang Shumin
Changchun University of Traditional Chinese Medicine, Changchun, Jilin, 130117, China.
Inflammation. 2016 Apr;39(2):928-37. doi: 10.1007/s10753-016-0326-2.
The aim of this study is to evaluate the protective effects of chrysophanol (CH) against paraquat (PQ)-induced pulmonary injury. Fifty BALB/C mice were randomized into five groups: (1) control, (2) PQ, (3) PQ + dexamethasone (Dex, 2 mg/kg), (4) PQ + CH (10 mg/kg), and (5) PQ + CH (20 mg/kg). A single dose of PQ (50 mg/kg, i.p.) was intraperitoneally given to induce acute lung injury. Then mice were treated with CH (10 and 20 mg/kg/day, orally) for 7 days. At the end of the experiment, animals were euthanized and then bronchoalveolar lavage fluid (BALF) and lung tissues were collected for histological observation, biochemical analysis, and Western blot analysis. Malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) levels in BALF were determined. The levels of SOD and MDA in the lung were also detected. The peroxisome proliferator-activated receptor (PPAR)-γ and nuclear factor-kappaB (NF-κB) pathway proteins in the lung were determined by Western blot. Histological examination indicated that CH attenuated lung inflammation caused by PQ. Biochemical results showed that CH treatment significantly reduced the levels of MDA, MPO, and inflammatory cytokines and increased the level of SOD, compared to those in the PQ group. Meanwhile, Western Blot results revealed that CH increased PPAR-γ expression and inhibited NF-κB pathway activation after PQ challenge. These findings suggested the potential therapeutic effects of CH which is derived from a natural product on PQ-induced pulmonary injury.
本研究旨在评估大黄酚（CH）对百草枯（PQ）诱导的肺损伤的保护作用。将50只BALB/C小鼠随机分为五组：（1）对照组，（2）PQ组，（3）PQ + 地塞米松（Dex，2 mg/kg）组，（4）PQ + CH（10 mg/kg）组，以及（5）PQ + CH（20 mg/kg）组。腹腔注射单剂量PQ（50 mg/kg）以诱导急性肺损伤。然后小鼠口服CH（10和20 mg/kg/天），持续7天。实验结束时，对动物实施安乐死，随后收集支气管肺泡灌洗液（BALF）和肺组织用于组织学观察、生化分析和蛋白质印迹分析。测定BALF中丙二醛（MDA）、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、白细胞介素-6（IL-6）、IL-1β和肿瘤坏死因子-α（TNF-α）的水平。还检测了肺中SOD和MDA的水平。通过蛋白质印迹法测定肺中过氧化物酶体增殖物激活受体（PPAR）-γ和核因子-κB（NF-κB）信号通路蛋白。组织学检查表明，CH减轻了PQ引起的肺部炎症。生化结果显示，与PQ组相比，CH治疗显著降低了MDA、MPO和炎性细胞因子的水平，并提高了SOD的水平。同时，蛋白质印迹结果显示，PQ攻击后CH增加了PPAR-γ的表达并抑制了NF-κB信号通路的激活。这些发现提示了源自天然产物的CH对PQ诱导的肺损伤具有潜在的治疗作用。
