Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, 90 Chilam-dong, Jinju 660-702, Republic of Korea ; The Fifth Hospital of Shijizhuang, Shijizhuang 050021, China.
Evid Based Complement Alternat Med. 2013;2013:503043. doi: 10.1155/2013/503043. Epub 2013 Jun 20.
Recently we have demonstrated that anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) have anticancer effects. Here, we investigate the effects of AIMs on cell proliferation and invasion as well as epithelial-mesenchymal transition (EMT) which have been linked to cancer metastasis in human uterine cervical cancer HeLa cells. AIMs inhibited the invasion of HeLa cells in a dose-dependent manner. AIMs inhibited MMP-9 expression in a dose-dependent manner. AIMs inhibited the motility of HeLa cells in a wound healing test. AIMs still suppressed NF- κ B activation induced by TNF. AIMs also inhibited EMT in HeLa cells. AIMs suppressed vimentin, N-cadherin, and β-catenin expression and induced E-cadherin. AIMs also suppressed expression of β-catenin and Snail, which was regulated by GSK-3. These effects of AIMs were also limited in the HeLa cells treated with TNF. In conclusion, this study indicates that AIMs have anticancer effects by suppressing NF- κ B-regulated genes and EMT, which relates to suppression of I κ B α phosphorylation and GSK-3 activity, respectively. However, the effects of AIMs were attenuated in the TNF-high condition.
最近,我们已经证明了葡萄(Vitis coignetiae Pulliat)果实中的花色苷(AIMs)具有抗癌作用。在这里,我们研究了 AIMs 对人宫颈癌 HeLa 细胞增殖、侵袭和上皮间质转化(EMT)的影响,因为这些过程与癌症转移有关。AIMs 呈剂量依赖性地抑制 HeLa 细胞的侵袭。AIMs 呈剂量依赖性地抑制 MMP-9 的表达。AIMs 在划痕愈合试验中抑制了 HeLa 细胞的迁移。AIMs 仍然抑制了 TNF 诱导的 NF-κB 激活。AIMs 还抑制了 HeLa 细胞中的 EMT。AIMs 抑制波形蛋白、N-钙黏蛋白和β-连环蛋白的表达,并诱导 E-钙黏蛋白的表达。AIMs 还抑制了由 GSK-3 调节的β-连环蛋白和 Snaill 的表达。这些 AIMs 的作用在 TNF 处理的 HeLa 细胞中也受到限制。总之,这项研究表明,AIMs 通过抑制 NF-κB 调节的基因和 EMT 发挥抗癌作用,这分别与抑制 IκBα磷酸化和 GSK-3 活性有关。然而,在 TNF 高表达的情况下,AIMs 的作用会减弱。