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解毒三参汤通过抑制上皮-间质转化抑制结肠癌SW480细胞的迁移和侵袭。

Jiedu Sangen Decoction Inhibits Migration and Invasion of Colon Cancer SW480 Cells via Suppressing Epithelial Mesenchymal Transition.

作者信息

Zhang Kai, Peng Tao, Yan Qingying, Sun Leitao, Miao Haojun, Yuan Li, Hong Yuxin, Zhou Mengmeng, Guo Kaibo, Zhang Leyin, Fang Jing, Shen Minhe, Ruan Shanming

机构信息

The First Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 310053, China.

Human Oncology and Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York 10065, USA.

出版信息

Evid Based Complement Alternat Med. 2018 Sep 25;2018:1495768. doi: 10.1155/2018/1495768. eCollection 2018.

DOI:10.1155/2018/1495768
PMID:30356400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6176311/
Abstract

Jiedu Sangen Decoction (JSD), a traditional Chinese medicine (TCM) formula, has been widely used in China to treat gastrointestinal cancer, especially as an adjuvant therapy in colorectal cancer (CRC) patients. This study aimed to evaluate the efficacy of JSD and Jiedu Sangen aqueous extract (JSAE) in colon cancer cells and explored the underlining mechanisms by cytotoxicity assay, scratch assay, transwell migration assay, matrigel invasion assay, confocal laser scanning microscopy, and western blot analysis. We demonstrated that JSAE inhibited the growth of colon cancer SW480 cells in a dose-dependent manner and JSAE repressed cancer cell migration and invasion. Furthermore, epithelial mesenchymal transition (EMT) was reversed by JSAE via enhancing E-cadherin expression and attenuating protein levels of EMT promoting factors such as N-cadherin, Slug, and ZEB1. These findings provided the first experimental evidence confirming the efficacy of JSAE in repressing invasion and metastasis of CRC and paving a way for the broader use of JSD in clinic.

摘要

解毒三根汤（JSD）是一种中药配方，在中国已被广泛用于治疗胃肠道癌症，尤其是作为结直肠癌（CRC）患者的辅助治疗。本研究旨在评估JSD和解毒三根水提取物（JSAE）对结肠癌细胞的疗效，并通过细胞毒性试验、划痕试验、Transwell迁移试验、基质胶侵袭试验、共聚焦激光扫描显微镜和蛋白质印迹分析来探讨其潜在机制。我们证明JSAE以剂量依赖性方式抑制结肠癌细胞SW480的生长，并且JSAE抑制癌细胞的迁移和侵袭。此外，JSAE通过增强E-钙黏蛋白的表达并降低EMT促进因子（如N-钙黏蛋白、Slug和ZEB1）的蛋白水平来逆转上皮-间质转化（EMT）。这些发现提供了首个实验证据，证实了JSAE在抑制CRC侵袭和转移方面的疗效，并为JSD在临床上的更广泛应用铺平了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/d886ec96c053/ECAM2018-1495768.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/e4d000146c34/ECAM2018-1495768.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/46f5f788fa4b/ECAM2018-1495768.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/6013285f9708/ECAM2018-1495768.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/e8505c1ce11f/ECAM2018-1495768.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/e612b1a60f68/ECAM2018-1495768.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/d886ec96c053/ECAM2018-1495768.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/e4d000146c34/ECAM2018-1495768.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/46f5f788fa4b/ECAM2018-1495768.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/6013285f9708/ECAM2018-1495768.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/e8505c1ce11f/ECAM2018-1495768.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/e612b1a60f68/ECAM2018-1495768.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0513/6176311/d886ec96c053/ECAM2018-1495768.006.jpg

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