Hellwig U
Blood Transfusion Centre, Berlin, DDR.
Biomed Biochim Acta. 1990;49(2-3):S340-3.
Desferrioxamine (DFO) in therapeutically useful amounts was incorporated into red blood cells (RBC) by means of a dialysis method. Entrapped DFO interacted with hemoglobin which was indicated by the formation of methemoglobin. This process was influenced to a great extent by the duration and temperature of the incubation steps. In contrast to added methylene blue the co-entrapment of ascorbic acid proved to be a very effective measure for protecting hemoglobin from oxidation and maintaining intracellular 2,3-diphosphoglycerate (2,3-DPG). Hypernormal values of 2,3-DPG could be obtained by the incubation in solutions containing phosphate, inosine, and pyruvate. Loaded RBC prepared in such a way should be characterized by a nearly normal oxygen-transport function and survival in vivo.
通过透析法将治疗有效量的去铁胺（DFO）掺入红细胞（RBC）中。包封的DFO与血红蛋白相互作用，高铁血红蛋白的形成表明了这一点。该过程在很大程度上受孵育步骤的持续时间和温度影响。与添加的亚甲蓝相比，抗坏血酸的共包封被证明是保护血红蛋白免受氧化和维持细胞内2,3 - 二磷酸甘油酸（2,3 - DPG）的非常有效的措施。通过在含有磷酸盐、肌苷和丙酮酸的溶液中孵育可获得2,3 - DPG的超常值。以这种方式制备的负载红细胞应以几乎正常的氧运输功能和体内存活率为特征。
