Dawson R B, Hershey R T, Myers C S
Am J Clin Pathol. 1978 May;69(5):505-8. doi: 10.1093/ajcp/69.5.505.
Methylene blue and inosine have been shown to stimulate glycolytic metabolism in the erythrocytes, increasing the concentration of 2.3-diphosphoglycerate (2,3-DPG), which is necessary for hemoglobin function, by regulating oxidative metabolism and providing a five-carbon nutrient for glycolysis, respectively. However, a recent study suggested that the methylene blue effect was dependent on the presence of inosine. This study was designed to establish, if possible, the existence of a methylene blue effect and to confirm the usefulness of inosine. The optimal concentration of inosine for increasing 2,3-DPG synthesis in a CPD-adenine preservative is confirmed to be 10--15 mM. Concentrations of 2,3-DPG were maintained in the erythrocytes at normal or higher levels for 21 days of storage with 10 or 15 mM inosine, whether the methylene blue was present or not. However, when methylene blue was present, 2,3-DPG concentrations were significantly better maintained.
亚甲蓝和肌苷已被证明可刺激红细胞中的糖酵解代谢，分别通过调节氧化代谢和为糖酵解提供五碳营养物质，提高对血红蛋白功能至关重要的2,3-二磷酸甘油酸（2,3-DPG）的浓度。然而，最近的一项研究表明，亚甲蓝的作用依赖于肌苷的存在。本研究旨在尽可能确定亚甲蓝作用的存在，并证实肌苷的效用。已证实，在CPD-腺嘌呤保存液中，用于增加2,3-DPG合成的肌苷最佳浓度为10-15 mM。无论是否存在亚甲蓝，在含有10或15 mM肌苷的情况下储存21天，红细胞中的2,3-DPG浓度都能维持在正常或更高水平。然而，当存在亚甲蓝时，2,3-DPG浓度能得到显著更好的维持。
