大黄素通过内在的线粒体和外在的死亡受体途径诱导人宫颈癌 hela 细胞凋亡。

Emodin induces apoptosis of human cervical cancer hela cells via intrinsic mitochondrial and extrinsic death receptor pathway.

机构信息

Department of Gynecology, First Affiliated Hospital of Heilongjiang University of Chinese Medcine, Hapin Road, 150040, Haerbin, Heilongjiang Province, China.

出版信息

Cancer Cell Int. 2013 Jul 16;13(1):71. doi: 10.1186/1475-2867-13-71.

BACKGROUND

Emodin is a natural anthraquinone derivative isolated from the Rheum palmatum L.

AIM

The aim of the present study was to investigate the effect of emodin on the apoptosis of the human cervical cancer line HeLa and to identify the mechanisms involved.

METHODS

Relative cell viability was assessed by MTT assay after treatment with emodin. Cell apoptosis was detected with TUNEL, Hoechst 33342 staining and quantified with flow cytometry using annexin FITC-PI staining.

RESULTS

The percentage of apoptotic cells was 0.8, 8.2, 22.1, and 43.7%, respectively. The mRNA levels of Caspase-9, -8 and -3 detected by Real-time PCR after treatment with emodin were significantly increased. Emodin increased the protein levels of Cytochome c, Apaf-1, Fas, FasL, and FADD but decreased the protein levels of Pro-caspase-9, Pro-caspase-8 and Pro-caspase-3.

CONCLUSION

We conclude that the emodin inhibited HeLa proliferation by inducing apoptosis through the intrinsic mitochondrial and extrinsic death receptor pathways.

背景

大黄素是从大黄（Rheum palmatum L.）中分离得到的一种天然蒽醌衍生物。

目的

本研究旨在探讨大黄素对人宫颈癌 HeLa 细胞凋亡的影响，并探讨其作用机制。

方法

用 MTT 法检测大黄素处理后细胞相对活力。用 TUNEL、Hoechst 33342 染色法检测细胞凋亡，用 Annexin FITC-PI 染色法通过流式细胞术定量分析。

结果

大黄素处理后细胞凋亡率分别为 0.8%、8.2%、22.1%和 43.7%。用大黄素处理后，实时 PCR 检测到 Caspase-9、-8 和 -3 的 mRNA 水平明显升高。大黄素增加了 Cytochome c、Apaf-1、Fas、FasL 和 FADD 的蛋白水平，但降低了 Pro-caspase-9、Pro-caspase-8 和 Pro-caspase-3 的蛋白水平。