Bulić-Jakus F, Skreb N, Jurić-Lekić G, Svajger A
Institute of Biology Medical Faculty, University of Zagreb, Yugoslavia.
Int J Dev Biol. 1990 Jun;34(2):275-9.
Rat egg cylinders at the primitive streak stage were grown in modified organ culture for 2 weeks using a chemically-defined medium. The purpose of the experiment was to determine whether the terminal tissue differentiation is modified by human transferrin. The control sets were grown in medium with or without rat serum. In explants treated with transferrin, groups of atypical cells of the ocular lens (lentoids) appeared more frequently than in both control sets; however neuroblasts were observed as often as in the serum-supplemented medium. Bovine serum albumin (BSA) stimulated the differentiation of neuroblasts but did not promote lentoid formation. We conclude that human transferrin does stimulate the differentiation of lentoids in rat embryonic explants, but the mechanism of its action remains unknown.
处于原条期的大鼠卵圆柱在改良器官培养中使用化学成分明确的培养基培养2周。该实验的目的是确定终末组织分化是否会被人转铁蛋白改变。对照组在添加或不添加大鼠血清的培养基中培养。在用转铁蛋白处理的外植体中，眼晶状体的非典型细胞群（类晶状体）出现的频率比两个对照组都高；然而，神经母细胞的观察频率与添加血清的培养基中一样。牛血清白蛋白（BSA）刺激了神经母细胞的分化，但没有促进类晶状体的形成。我们得出结论，人转铁蛋白确实能刺激大鼠胚胎外植体中类晶状体的分化，但其作用机制仍然未知。
