Skreb N, Bulic-Jakus F, Crnek V, Stepic J, Vlahovic M
Institute of Biology, Faculty of Medicine, Zagreb, Republic of Croatia.
Int J Dev Biol. 1993 Mar;37(1):151-4.
Modified organ cultures of rat egg-cylinders were grown for 2 weeks in Eagle's minimal essential medium (MEM) without serum. Differentiation of epidermis and cartilage in the cultures deprived of serum was comparable to that in fully serum-supplemented medium, whereas other differentiated tissues were rare or absent. The purpose of the experiment was to determine whether terminal tissue differentiation is modified by various added factors. The factors used affected the growth and/or differentiation of explants as follows: bovine serum albumin and human transferrin had a positive permissive influence on the appearance of neuroblasts; human transferrin alone stimulated the formation of lentoids, a relatively rare tissue. Retinoic acid inhibited cartilage formation and stimulated the differentiation of cylindrical epithelium; neural growth factor inhibited the growth of explants; and 5-azacytidine impeded the survival of explants. One can conclude that these factors influenced the growth and differentiation of the early rat embryos cultured in a chemically defined medium.
将大鼠卵圆柱的改良器官培养物在不含血清的伊格尔基本培养基（MEM）中培养2周。在无血清培养物中，表皮和软骨的分化与完全补充血清的培养基中的情况相当，而其他分化组织则很少见或不存在。该实验的目的是确定终末组织分化是否会受到各种添加因子的影响。所使用的因子对植块的生长和/或分化的影响如下：牛血清白蛋白和人转铁蛋白对成神经细胞的出现有积极的许可作用；单独的人转铁蛋白刺激类晶状体的形成，类晶状体是一种相对罕见的组织。视黄酸抑制软骨形成并刺激柱状上皮的分化；神经生长因子抑制植块的生长；5-氮杂胞苷阻碍植块的存活。可以得出结论，这些因子影响了在化学成分明确的培养基中培养的早期大鼠胚胎的生长和分化。
