Stipić J, Skreb F, Bradamante Z, Skreb N
Clinic of Neurology, University Hospital, Zagreb, Republic of Croatia, Yugoslavia.
Int J Dev Biol. 1991 Sep;35(3):203-7.
Rat egg-cylinders at the primitive streak stage were grown in modified organ culture for 2 weeks using a chemically defined medium. Differentiation of the epidermis and cartilage was comparable to that in fully serum-supplemented medium, whereas neuroblasts were very scarce. In explants treated either with bovine serum albumin or transferrin, neuroblasts were observed, whereas the addition of NGF did not improve neuroblast differentiation. On the contrary, NGF impaired growth and tissue differentiation when compared with explants grown in serum-free medium.
将处于原条期的大鼠卵圆柱体在改良器官培养中使用化学成分明确的培养基培养2周。表皮和软骨的分化与在完全补充血清的培养基中的情况相当，而成神经细胞非常稀少。在用牛血清白蛋白或转铁蛋白处理的外植体中观察到了成神经细胞，而添加神经生长因子（NGF）并未改善成神经细胞的分化。相反，与在无血清培养基中生长的外植体相比，NGF损害了生长和组织分化。
