Department of Medical Specialities, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain,
Department of Medical Specialities, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Faculty of Medicine, Alcalá University, Madrid, Spain.
Histol Histopathol. 2014 Jan;29(1):139-49. doi: 10.14670/HH-29.139. Epub 2013 Jul 19.
Despite their degradation in the host organism, the benefits of collagen bioprostheses remain unclear. This study addresses the absorption and long-term host tissue incorporation of several collagen biomeshes.
Partial ventral hernial defects created in the abdominal wall of rabbits were repaired using the crosslinked meshes Permacol® or CollaMend®, or the non-crosslinked Surgisis®, Tutomesh® or Strattice®. After 90 and 180 days of implant, morphological studies and morphometric analysis of the thickness of the meshes were performed. Immunofluorescence confocal microscopy combined with differential interference contrast (DIC) imaging was used to distinguish newly formed collagen from that comprising the mesh. The macrophage response was examined by immunohistochemistry.
At 90 days, the thinner non-crosslinked biomeshes Tutomesh and Surgisis were more fully degraded with much of their collagen replaced with loose connective tissue. By 180 days, both implants had been practically fully absorbed. In contrast, in Strattice only the outermost third was infiltrated by neoformed tissue. On both surfaces of the crosslinked meshes, a fibrous capsule with host cells lining its perimeter was observed at both time points, though at 180 days these cells had penetrated the mesh interior. At both implant times, Strattice showed the higher expression of collagen type I while collagen III expression was similar for all the meshes. The non-crosslinked materials elicited lower macrophage counts at both time points, significantly so for Strattice. The macrophage response decreased over time for all the meshes but Surgisis.
Strattice, the thicker, more compacted non-crosslinked mesh showed the best balance between tissue incorporation and absorption while eliciting a minimal foreign-body reaction in the long-term.
尽管胶原蛋白生物假体在宿主组织中发生降解，但它们的益处仍不清楚。本研究旨在探讨几种胶原蛋白生物网片的吸收和长期宿主组织整合情况。
在兔腹壁上造成部分腹侧疝缺损，使用交联的 Permacol®或 CollaMend®网片、非交联的 Surgisis®、Tutomesh®或 Strattice®进行修复。在植入后 90 天和 180 天，进行形态学研究和网片厚度的形态计量学分析。免疫荧光共聚焦显微镜结合相差对比（DIC）成像用于区分新形成的胶原蛋白和构成网片的胶原蛋白。通过免疫组织化学检测巨噬细胞反应。
在 90 天时，较薄的非交联生物网片 Tutomesh 和 Surgisis 降解更充分，其大部分胶原蛋白被疏松结缔组织取代。到 180 天时，两种植入物几乎已完全被吸收。相比之下，在 Strattice 中只有最外层的三分之一被新生组织浸润。在交联网片的两面，在两个时间点都观察到有纤维囊，其周边有宿主细胞排列，尽管在 180 天时这些细胞已穿透网片内部。在两个植入时间点，Strattice 均显示出更高的 I 型胶原蛋白表达，而所有网片的 III 型胶原蛋白表达相似。在两个时间点，非交联材料的巨噬细胞计数均较低，Strattice 则更为显著。所有网片的巨噬细胞反应随时间减少，但 Surgisis 除外。
Strattice 是一种更厚、更致密的非交联网片，在长期情况下，在组织整合和吸收之间表现出最佳平衡，同时引发最小的异物反应。
