Ebert T, Bander N H, Finstad C L, Ramsawak R D, Old L J
Laboratory of Human Cancer Immunology, Memorial Sloan-Kettering Cancer Center, New York, New York.
Cancer Res. 1990 Sep 1;50(17):5531-6.
We have reviewed our laboratory's efforts to establish continuous human renal cancer cell lines. During the 16-year period of 1972 through 1987, 498 successive attempts resulted in establishment of 63 renal cancer cell lines. Of these lines, 46 were derived from primary kidney tumors and 17 from metastatic sites (lung, brain, bone, and lymph node). Forty-three of these lines have been characterized with regard to morphology, growth kinetics, anchorage-independent growth, tumorigenicity in athymic nude mice, and expression of kidney cell surface antigens. These results were compared with data from primary short term cultures of normal kidney epithelium. The overall success rate of establishing continuous renal cancer cell lines was 12.7%. In general, no significant difference in success was noted based on whether the specimen was derived from a primary or a metastatic lesion. However, all successfully established lines were derived from tumors exhibiting clinically "aggressive" behavior. All cell lines expressed proximal tubular cell differentiation antigens. Significant morphological heterogeneity was observed among normal kidney as well as kidney cancer cell lines in vitro. No significant difference in doubling time was found between cell lines of renal cancer and passage 1 cultures of normal kidney epithelium. Twenty-one of 30 (70%) lines assayed formed clones on soft agar and 26 of 33 (79%) lines grew in athymic mice. Among the 25 lines which were assayed for both soft agar growth and tumorigenicity in nude mice, this pair of phenotypic traits were concordant in 17 lines (60%). Four lines (16%) grew on agar but not in mice, while four other lines (16%) failed to grow in agar but were tumorigenic in mice.
我们回顾了本实验室建立人肾癌连续细胞系的工作。在1972年至1987年的16年间，498次连续尝试成功建立了63个人肾癌细胞系。其中，46个细胞系源自原发性肾肿瘤，17个源自转移部位（肺、脑、骨和淋巴结）。已对其中43个细胞系的形态学、生长动力学、非贴壁依赖性生长、无胸腺裸鼠致瘤性以及肾细胞表面抗原表达进行了表征。将这些结果与正常肾上皮细胞原代短期培养的数据进行了比较。建立连续肾癌细胞系的总体成功率为12.7%。一般而言，根据标本是源自原发性还是转移性病变，成功率没有显著差异。然而，所有成功建立的细胞系均源自表现出临床“侵袭性”行为的肿瘤。所有细胞系均表达近端肾小管细胞分化抗原。在体外，正常肾细胞系和肾癌细胞系之间观察到显著的形态学异质性。肾癌细胞系与正常肾上皮细胞第1代培养物之间的倍增时间没有显著差异。在检测的30个细胞系中，有21个（70%）在软琼脂上形成克隆，在检测的33个细胞系中，有26个（79%）在无胸腺小鼠中生长。在同时检测软琼脂生长和裸鼠致瘤性的25个细胞系中，这一对表型特征在17个细胞系（60%）中是一致的。4个细胞系（16%）在琼脂上生长但在小鼠中不生长，而另外4个细胞系（16%）在琼脂上不生长但在小鼠中具有致瘤性。
