INRA, UMR1213 Herbivores, F-63122 St-Genès-Champanelle, France.
Food Chem. 2013 Dec 1;141(3):1888-94. doi: 10.1016/j.foodchem.2013.04.072. Epub 2013 May 3.
The aim of this work was to characterise new UV-absorbing compounds (UAC) in cow milk in order to gain an overview of the molecular diversity of the minor bioactive constituents, that could be used to trace animal feed or that potentially affect milk quality. UAC were extracted from lyophilized milks, partitioned using SPE C-18 cartridges, purified by semi-preparative HPLC then analysed by HPLC/DAD/HRMS in both ESI(-) and ESI(+) ionisation mode. Compounds that remained unidentified after comparison with UV and MS databases were analysed by 1D and 2D NMR techniques. The identification and structural elucidation of N-cinnamoylglycine, 2,4-, 2,6-, 2,8-quinolinediols, and 1-methyl-3-carboxy-beta-carboline are described.
本工作旨在对牛奶中的新型紫外吸收化合物（UAC）进行表征，以全面了解可能用于追踪动物饲料或潜在影响牛奶质量的微量生物活性成分的分子多样性。UAC 从冻干牛奶中提取，使用 SPE C-18 小柱进行分离，然后通过半制备 HPLC 进行纯化，最后通过 HPLC/DAD/HRMS 在 ESI(-)和 ESI(+)两种离子化模式下进行分析。与 UV 和 MS 数据库比较后仍无法鉴定的化合物，通过 1D 和 2D NMR 技术进行分析。描述了肉桂酰甘氨酸、2,4-、2,6-、2,8-邻苯二酚和 1-甲基-3-羧基-β-咔啉的鉴定和结构阐明。
