狼疮血清对Sm-D自身抗原合成肽的识别。
Recognition of synthetic peptides of Sm-D autoantigen by lupus sera.
作者信息
Barakat S, Briand J P, Weber J C, van Regenmortel M H, Muller S
机构信息
Immunochemistry Laboratory, Institute of Molecular and Cellular Biology, Strasbourg, France.
出版信息
Clin Exp Immunol. 1990 Aug;81(2):256-62. doi: 10.1111/j.1365-2249.1990.tb03327.x.
Abstract
The reactivity of autoantibodies present in the serum of patients with systemic lupus erythematosus (SLE) was investigated by ELISA using seven overlapping synthetic peptides representing the entire sequence of the polypeptide D component of 'Sm antigen'. Of the 165 SLE sera tested, 59% were found to contain IgG antibodies able to bind to peptide 1-20, while 37% of the sera reacted with peptide 44-67. All sera reacting with peptide 44-67 also reacted with peptide 1-20. These two peptides were only seldom recognized by the sera of 187 patients with other rheumatic autoimmune diseases or by 53 sera of normal individuals. In a parallel study using sera that reacted with the D band in immunoblotting, most of the sera recognized peptides 44-67 (89%) and 1-20 (67%), while 33% of them reacted with peptide 97-119. The use of these synthetic peptides in ELISA may be of considerable help for detecting anti Sm autoantibodies.
摘要
采用酶联免疫吸附测定（ELISA），利用代表“Sm抗原”多肽D组分完整序列的7个重叠合成肽，研究了系统性红斑狼疮（SLE）患者血清中自身抗体的反应性。在检测的165份SLE血清中，发现59%含有能与肽1 - 20结合的IgG抗体，而37%的血清与肽44 - 67发生反应。所有与肽44 - 67反应的血清也与肽1 - 20反应。这两个肽很少被187例其他风湿性自身免疫病患者的血清或53份正常人血清识别。在一项平行研究中，使用在免疫印迹中与D带反应的血清，大多数血清识别肽44 - 67（89%）和肽1 - 20（67%），而其中33%与肽97 - 119反应。在ELISA中使用这些合成肽可能对检测抗Sm自身抗体有很大帮助。
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