Ye X Y, Ng T B
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
J Pept Res. 2001 Apr;57(4):330-6. doi: 10.1046/j.1397-002x.2001.00874.x.
A protein designated hypogin, with a prominent suppressive action on the growth of the fungi Mycosphaerella arachidicola, Fusarium oxysporum and Coprinus comatus, was isolated from seeds of the peanut Arachis hypogaea. The protein inhibited human immunodeficiency virus (HIV) reverse transcriptase and enzymes associated with HIV infection including alpha-glucosidase and beta-glucosidase. The proliferative response of mouse splenocytes was attenuated in the presence of the protein. The protein exhibited a molecular mass of 7.2 kDa in tricine gel electrophoresis and gel filtration on Superdex 75 and an N-terminal sequence resembling peanut allergen Ara H1. The isolation procedure involved affinity chromatography on Affi-gel blue gel and ion-exchange chromatography on CM-Sepharose. The protein was adsorbed in both chromatographic media.
从花生（Arachis hypogaea）种子中分离出一种名为hypogin的蛋白质，它对落花生球腔菌（Mycosphaerella arachidicola）、尖孢镰刀菌（Fusarium oxysporum）和毛头鬼伞（Coprinus comatus）的真菌生长具有显著的抑制作用。该蛋白质可抑制人类免疫缺陷病毒（HIV）逆转录酶以及与HIV感染相关的酶，包括α-葡萄糖苷酶和β-葡萄糖苷酶。在该蛋白质存在的情况下，小鼠脾细胞的增殖反应减弱。在tricine凝胶电泳和Superdex 75凝胶过滤中，该蛋白质的分子量为7.2 kDa，其N端序列与花生过敏原Ara H1相似。分离过程包括在Affi-gel蓝凝胶上进行亲和色谱和在CM-琼脂糖凝胶上进行离子交换色谱。该蛋白质在两种色谱介质中均被吸附。
